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Assay Kits  >  Thrombin assay kits  >>  SensoLyte ® 520 Thrombin Activity Assay Kit *Fluorimetric*

Product Name SensoLyte ® 520 Thrombin Activity Assay Kit *Fluorimetric*
Size 1 kit
Catalog # AS-72129
US$ $501

Thrombin, a serine protease, plays a central role in hemostasis by converting soluble plasma fibrinogen into an insoluble fibrin clot and by promoting platelet aggregation. Thrombin also influences a number of normal and pathological processes, including inflammation, tissue repair, embryogenesis, angiogenesis, and tumor invasion.

The SensoLyte® 520 Thrombin Assay Kit is optimized for detecting thrombin activity. This kit contains a novel internally quenched 5-FAM/QXL® 520 FRET substrate for thrombin. Thrombin cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence which can be monitored at excitation/emission= 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assay can be performed in a convenient 96-well or 384-well microplate format for screening of enzyme inhibitors.

Kit size: 100 assays.

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
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Gugerell, A. et al. (2012). High thrombin concentrations in fibrin sealants induce apoptosis in human keratinocytes. J Biomed Mater Res A. 100A: 1239. doi: 10.1002/jbm.a.34007

Luan, NM. et al. (2011). Layer-by-layer co-immobilization of soluble complement receptor 1 and heparin on islets. Biomaterials. 32: 6487. doi:10.1016/j.biomaterials.2011.05.048

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Shiang, YC. et al. (2010). Aptamer-conjugated nanoparticles efficiently control the activity of thrombin. Adv Funct Mater 20, 3175. doi: 10.1002/adfm.201000642.
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