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Assay Kits  >  Thrombin assay kits  >>  SensoLyte ® AFC Thrombin Activity Assay Kit *Fluorimetric*

Product Name SensoLyte ® AFC Thrombin Activity Assay Kit *Fluorimetric*
Size 1 kit
Catalog # AS-72130
US$ $434

Thrombin, a serine protease, plays a central role in hemostasis by converting soluble plasma fibrinogen into an insoluble fibrin clot and by promoting platelet aggregation. Thrombin also influences a number of normal and pathological processes, including inflammation, tissue repair, embryogenesis, angiogenesis, and tumor invasion.

The SensoLyteŽ AFC Thrombin Assay Kit is optimized for screening of enzyme inhibitors. This kit contains a fluorogenic substrate which shows high reactivity and low background. Thrombin cleaves the substrate resulting in the release of the AFC (7-amido-4-trifluoromethylcoumarin) fluorophore. Fluorescence can be monitored at excitation/emission= 380 nm/500 nm. The assays are performed in a convenient 96-well or 384-well microplate format.

Kit size: 100 assays.

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Product Citations Ambadapadi, S. et al. (2015) Reactive Center Loop (RCL) peptides derived from serpins display independent coagulation and immune modulating activities J. Biol. Chem. 291 2874-2887 doi: 10.1074/jbc.M115.704841

Nair, DG. et al. (2014). Interactions of some commonly used drugs with Human α-thrombin. J Biomol Struct Dyn doi: 10.1080/07391102.2014.923329.

Wendeler, M. et al. (2014). Process-scale purification and analytical characterization of highly gamma-carboxylated recombinant human prothrombin. J Chromatogr A 1325, 171.

Yang, H. et al. (2013). Interleukin-1 promotes coagulation, which Is necessary for protective immunity in the lung against Streptococcus pneumoniae infection. J Infect Dis 207, 50. doi: 10.1093/infdis/jis651.

Sasaki, T. et al. (2012). Intravenous and oral administrations of DD2 [7-Amino-2-(sulfanylmethyl)heptanoic acid] produce thrombolysis through inhibition of plasma TAFIa in rats with tissue factor-induced microthrombosis. Thrombosis Res 130, e222. doi:10.1038/jid.2012.204

Lim, HA. et al. (2011). Novel agmatine and agmatine-like peptidomimetic inhibitors of the West Nile Virus NS2B/NS3 serine protease. Eur J Med Chem 10.1016/j.ejmech.2011.04.055.
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