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Assay Kits  >  Renin Assay Kits  >>  SensoLyte ® 520 Mouse Renin Assay Kit *Fluorimetric*

Product Name SensoLyte ® 520 Mouse Renin Assay Kit *Fluorimetric*
Size 1 kit
Catalog # AS-72161
US$ $611

Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I. Angiotensin I is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood vessels leading to increased blood pressure. Discovery of new renin inhibitors for treatment of hypertension is a promising area of research.

The SensoLyte® 520 Mouse Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of mouse renin activity using a 5-FAM/QXL®520 FRET peptide. In the FRET peptide the fluorescence of 5-FAM is quenched by QXL®520. Upon cleavage into two separate fragments by renin, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission = 490/520 nm. The kit contains: 5-FAM/QXL®520-based FRET mouse specific renin substrate (Ex/Em=490/520 nm upon cleavage), assay buffer, mouse recombinant prorenin, reagents for conversion of prorenin to renin, inhibitor, fluorescence reference standard for calibration, and an optimized assay protocol.

100 assays (96-well plate)

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Product Citations Kidokoro, K. et al. (2016). Feasibility of fluorescence energy transfer system for imaging the renoprotective effects of aliskiren in diabetic mice. J.Renin-Angiotensin-Aldosterone Sys. doi.org/10.1177/1470320315625704

Harlan SM et al. (2015). Viral transduction of renin rapidly establishes persistent hypertension in diverse murine strains. AM J Physiol, 309(5) R467 doi: 10.1152/ajpregu.00106.2015

Kong, J. et al. (2013). VDR Attenuates Acute Lung Injury by Blocking Ang-2-Tie-2 Pathway and Renin-Angiotensin System. Mol Endocrinol 27, 2116. doi: 10.1210/me.2013-1146.

Kakoki, M. et al. (2013). Primary aldosteronism and impaired natriuresis in mice underexpressing TGFβ1. PNAS 110, 5600. doi: 10.1073/pnas.1302641110.

Takada, S. et al. (2013). Angiotensin II receptor blocker improves the lowered exercise capacity and impaired mitochondrial function of the skeletal muscle in type 2 diabetic mice. J Appl Physiol 114, 844. doi: 10.​1152/​japplphysiol.​00053.​2012.

Prysyazhna, O. et al. (2012). Single atom substitution in mouse protein kinase G eliminates oxidant sensing to cause hypertension. Nature Med 18, 286.

Tsukuda, K. et al. (2012). Influence of angiotensin II type 1 receptor-associated protein on prenatal development and adult hypertension after maternal dietary protein restriction during pregnancy. J Am Soc Hypertension 6, 324. doi:10.1016/j.jash.2012.07.001

Wang, Y. et al. (2012). Vitamin D receptor signaling in podocytes protects against diabetic nephropathy. J Am Soc Nephrol doi:10.1681/ASN.2012040383.
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