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Assay Kits  >  Coagulation Assay Kits  >>  SensoLyte ® 520 Factor Xa Assay Kit *Fluorimetric*

Product Name SensoLyte ® 520 Factor Xa Assay Kit *Fluorimetric*
Size 1 kit
Catalog # AS-72208
US$ $501

Factor Xa (FXa) is a serine endopeptidase composed of two disulfide-linked subunits. FXa leads to blood clot formation by converting prothrombin to thrombin through the prothrombinase complex. FXa is generated from zymogen Factor X via the intrinsic and extrinsic pathways and is the rate-limiting step in the propagation of thrombin generation. In the presence of Ca2+ ions, FXa forms prothrombinase with factor Va on the phospholipid membrane of the activated platelets. FXa has emerged as an attractive target for drug discovery for thromboembolic diseases.

The SensoLyte® 520 Factor Xa Assay Kit provides a convenient assay for high throughput screening of FXa inhibitors or continuous assay of enzyme activity. It detects FXa activity using a 5-FAM/QXL® 520 fluorescence resonance energy transfer (FRET) peptide. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL® 520. Upon cleavage into two separate fragments by FXa, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission wavelengths = 490 nm/520 nm. With excellent fluorescence quantum yield and longer wavelength, 5-FAM shows less interference from autofluorescence of test compounds and cellular components. The assays are performed in a convenient 96-well microplate format.

Kit size: 100 assays

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Product Citations Lagos, F. C. et al. (2017). Novel FXa Inhibitor Identification through Integration of Ligand- and Structure-Based Approaches molecules 22 1588, doi:10.3390/molecules22101588
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