Labeling-detection

5-FAM [5-Carboxyfluorescein]

$261.00
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  • Cat.Number : AS-81003-1
  • Availability :
    In production

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Although FITC reagents have been more often used to prepare fluorescent bioconjugates, the low stability of FITC bioconjugates makes some researchers use amine-reactive succinimidyl esters of carboxyfluorescein (commonly called FAM) in bioconjugations. FAM reagents generate carboxamides that are more resistant to hydrolysis. We have shown that FAM reagents require less stringent reaction conditions, give better conjugation yields, and the resulted conjugates have superior stability. We noted that FITC labeled peptides tend to deteriorate more quickly than the corresponding FAM conjugates.
5-FAM is a single isomer. It is one of the most popular green fluorescent reagents used for in situ labeling peptides, proteins and nucleotides. It has also been used to prepare various small fluorescent molecules.

Specifications

Chemistry
CAS registry number
  • 76823-03-5
Molecular Formula
  • C21H12O7
Molecular Mass/ Weight
  • 376.3
Properties
Absorbance (nm)
  • 492
Emission (nm)
  • 518
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥95%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMF or DMSO
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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References

Comparison of Three Common Amine Reactive Fluorescent Probes Used for Conjugation to Biomolecules by Capillary Zone Electrophoresis

Bioconjug Chem . 1995 Jul 01 ; 6(4) 447 | DOI : https://doi.org/10.1021/bc00034a015

  • PR. Banks
  • D. Paquette

Cyanine Dyes with High Absorption Cross Section as Donor Chromophores in Energy Transfer Primers

Anal Biochem . 1996 Dec 01 ; 243(1) 15 | DOI : https://doi.org/10.1006/abio.1996.0477

  • SC. Hung
  • et al