Labeling-detection

5-TMRIA [Tetramethylrhodamine-5-iodoacetamide] - 5 mg

$267.00
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  • Cat.Number : AS-81410
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    In stock
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Quantity

Tetramethylrhodamine iodoacetamides (TMRIA) are thiol-selective reactive dyes that are used to label proteins via the cysteine residues. 5-TMRIA, the pure 5-isomer of TMRIA, is increasingly preferred for some particular applications since the mixed isomers of TMRIA may give different results from batch to batch due to the varying ratios and different reactivities of the two isomers. For example, 5-TAMRA is reported to predominantly label SH-1 (Cys-707) of the myosin heavy chain in skinned muscle fibers.

Specifications

Chemistry
CAS registry number
  • 114458-99-0
Molecular Formula
  • C26H24IN3O4
Molecular Mass/ Weight
  • 569.4
Properties
Absorbance (nm)
  • 541
Emission (nm)
  • 567
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥80%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMF or DMSO
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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References

Ca2+- and Cross-Bridge-Dependent Changes in N- and C-Terminal Structure of Troponin C in Rat Cardiac Muscle

Biophys J . 2001 Jan 01 ; 80(1) 360 | DOI : https://doi.org/10.1016/S0006-3495(01)76020-1

  • DA. Martyn
  • et al

Conformation of xanthene dyes in the sulfhydryl 1 binding site of myosin. 2

Biochemistry . 1995 Dec 12 ; 34 15943 | DOI : 10.1021/bi00049a009

  • K. Ajtai
  • TP. Burghardt