Labeling-detection

5(6)-FAM [5-(and-6)-Carboxyfluorescein] UltraPure Grade Mixed Isomers

$131.00
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  • Cat.Number : AS-81002-1
  • Availability :
    In production

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Although FITC reagents have been more often used to prepare fluorescent bioconjugates, the low stability of FITC bioconjugates makes some researchers use amine-reactive succinimidyl esters of carboxyfluorescein (commonly called FAM) in bioconjugations. FAM reagents generate carboxamides that are more resistant to hydrolysis. We have shown that FAM reagents require less stringent reaction conditions, give better conjugation yields, and the resulted conjugates have superior stability. We noted that FITC labeled peptides tend to deteriorate more quickly than the corresponding FAM conjugates.

Specifications

Chemistry
CAS registry number
  • 76823-03-5
Molecular Formula
  • C21H12O7
Molecular Mass/ Weight
  • 376.3
Properties
Absorbance (nm)
  • 494
Emission (nm)
  • 519
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥95%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMF or DMSO
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Measurement of flow velocity and inference of liquid viscosity in a microfluidic channel by fluorescence photobleaching.

Langmuir . 2014 Apr 14 ; 30(16) 4868 | DOI : 10.1021/la404891g

  • N.J. Carroll

References

A long lifetime chemical sensor: study on fluorescence property of fluorescein isothiocyanate and preparation of pH chemical sensor

Spectrochim Acta A Mol Biomol Spectrosc . 2004 Jul 01 ; 60(8-9) 1865 | DOI : https://doi.org/10.1016/j.saa.2003.10.004

  • LY Ma
  • et al

A rapid method for measuring intracellular pH using BCECF-AM

Biochim Biophys Acta . 2002 Aug 15 ; 1572(1) 143 | DOI : https://doi.org/10.1016/S0304-4165(02)00303-3

  • P. Ozkan
  • et al

In Vivo Application of Intestinal pH Measurement Using 2,7‘-Bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) Fluorescence Imaging

Photochem Photobiol . 1999 Nov 01 ; 70(5) 813 | DOI : https://doi.org/10.1111/j.1751-1097.1999.tb08287.x

  • X. Marechal
  • et al

Quenching of fluorescence of 1-hydroxypyrene-3,6,8-trisulfonate (HPTS) by Cu2+, Co2+, Ni2+, I−, and cetylpyridinium (CP+) ions in water/AOT/heptane microemulsion

J Colloid Interface Sci . 2004 Mar 03 ; 271(1) 157 | DOI : https://doi.org/10.1016/j.jcis.2003.09.038

  • S. Biswas

Rapid method for the preparation of a robust optical pH sensor

Analyst . 2003 Aug 19 ; 128 1181 | DOI : https://doi.org/10.1039/B304902G

  • HR. Kermis
  • et al