Labeling-detection

5(6)-TAMRA-X, SE [6-(Tetramethylrhodamine-5-(and-6)-carboxamido) hexanoic acid, SE] - 5 mg

$133.00
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  • Cat.Number : AS-81127
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TAMRA-X contains a seven-atom aminohexanoyl spacer (known as ‘X’) between TAMRA fluorophore and the succinimidyl ester. The ‘X’ spacer separates the fluorophore from the biomolecule to which it is conjugated, potentially reducing the quenching that typically occurs upon conjugation. We recommend this TAMRA-X derivative as the preferred dye for preparing TAMRA-labeled proteins when the fluorescence quenching of the labeling dye by protein is a serious problem.

Specifications

Chemistry
CAS registry number
  • 527687-02-1
Molecular Formula
  • C35H36N4O8
Molecular Mass/ Weight
  • 640.7
Properties
Absorbance (nm)
  • 544
Emission (nm)
  • 572
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥80%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMF or DMSO
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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References

Spectral Fluctuation of a Single Fluorophore Conjugated to a Protein Molecule

Biophys J . 2000 Mar 01 ; 78(3) 1561 | DOI : https://doi.org/10.1016/S0006-3495(00)76708-7

  • T. Wazawa
  • et al

Single Molecule Detection Technologies in Miniaturized High Throughput Screening: Fluorescence Correlation Spectroscopy

J Biomol Screen . 1999 Dec 01 ; 4(6) 335 | DOI : https://doi.org/10.1177/108705719900400609

  • KJ. Moore
  • et al