Labeling-detection

5(6)-TAMRA,SE [5-(and-6)-Carboxytetramethylrhodamine, SE]

$141.00
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  • Cat.Number : AS-81124
  • Availability :
    In production
  • Shipping conditions : Ice fees will apply

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Tetramethylrhodamine (TMR) is one of the fluorophores most often used for preparing peptide, protein, nucleotide and nucleic acid conjugates, especially fluorescent antibodies and avidin derivatives used in immunochemistry. TMR dyes have been widely used as acceptors for FAM fluorophores in a variety of FRET studies. The succinimidyl esters of 5-TAMRA, 6-TAMRA or the mixed isomers are the primary labeling reagents.

Specifications

Chemistry
CAS registry number
  • 150408-83-6
Molecular Formula
  • C29H25N3O7
Molecular Mass/ Weight
  • 527.5
Properties
Absorbance (nm)
  • 546
Emission (nm)
  • 575
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥90%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMF or DMSO
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Optical Properties and Application of a Reactive and Bioreducible Thiol-Containing Tetramethylrhodamine Dimer.

Bioconjugate Chem . 2009 Mar 18 ; 20(3) 476 | DOI : 10.1021/bc800367e

  • R. Christie

References

Genotyping Single-Nucleotide Polymorphisms by the Invader Assay with Dual-Color Fluorescence Polarization Detection

Clin Chem . 2001 Aug 01 ; 47(8) 1373 | DOI : https://doi.org/10.1093/clinchem/47.8.1373

  • TM. Hsu

Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis

Electrophoresis . 2001 Aug 01 ; 22(13) 2691 | DOI : 10.1002/1522-2683(200108)22:13<2691::AID-ELPS2691>3.0.CO;2-S

  • M. Hahn
  • et al