Labeling-detection

ADHP [10-Acetyl-3,7-dihydroxyphenoxazine] - 25 mg

$273.00
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  • Cat.Number : AS-85500
  • Availability :
    In production

Quantity

10-Acetyl-3,7-dihydroxyphenoxazine (ADHP), also called Amplex® Red and Ampliflu™ Red, is not only a sensitive and stable fluorogenic substrate for HRP but also an ultrasensitive probe for H2O2. In the presence of HRP and H2O2, ADHP generates highly fluorescent resorufin that has maximum absorption of 571 nm and maximum emission of 585 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of ADHP is minimal. So far ADHP has been known as the most sensitive and stable fluorogenic probe for detecting HRP and H2O2. ADHP has been widely used to detect HRP in many immunoassays. On the other hand, Zhou, et al. have demonstrated that ADHP can be used to detect trace amount of H2O2. The ADHP-based H2O2 detection is at least one order of magnitude more sensitive than the commonly used scopoletin assay for H2O2. Because H2O2 is produced in many enzymatic redox reactions, ADHP can be used in coupled enzymatic reactions to detect the activity of many oxidases and/or related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol, L-glutamate, amino acids, etc. We offer the best quality of ADHP with the most competitive price. The reagent can be purchased in a single 25 mg vial or can be custom-packaged to meet your special requirements.

Specifications

Chemistry
CAS registry number
  • 119171-73-2
Molecular Formula
  • C14H11NO4
Molecular Mass/ Weight
  • 257.3
Properties
Absorbance (nm)
  • 573
Emission (nm)
  • 590
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥95%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMSO
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

What are the sources of hydrogen peroxide production by heart mitochondria?

Biochim Biophys Acta . 2017 Feb 10 ; 1797(6-7) 939 | DOI : 10.1016/j.bbabio.2010.02.013

  • V. Grivennikova

On-chip microfluidic transport and bio/chemical sensing based on electrochemical bubble formation

Sensors and Actuators B: Chemical . 2007 May 21 ; 123(2) 1153 | DOI : 10.1016/j.snb.2006.10.028

  • W. Satoh

Determination of the activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase in a microfluidic system

Biosensors & Bioelectronics . 2007 Mar 01 ; 22(7) 1330 | DOI : 10.1016/j.bios.2006.06.007

  • N. Ohgami

Microprocessing of Liquid Plugs for Bio/chemical Analyses.

Anal Chem . 2008 Jun 16 ; 80(16) 6206 | DOI : 10.1021/ac800492v

  • F. Sassa

Autonomous Microfluidic Control by Chemically Actuated Micropumps and Its Application to Chemical Analyses.

Anal Chem . 2010 Jul 29 ; 82(16) 6870 | DOI : https://doi.org/10.1021/ac1009657

  • A. Takashima

References

A Fluorescence-Based, High-Throughput Sphingomyelin Assay for the Analysis of Niemann–Pick Disease and Other Disorders of Sphingomyelin Metabolism

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A fluorometric assay for detection of lysyl oxidase enzyme activity in biological samples

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A One-Step Fluorometric Method for the Continuous Measurement of Monoamine Oxidase Activity

Anal Biochem . 1997 Nov 15 ; 253(2) 169 | DOI : https://doi.org/10.1006/abio.1997.2392

  • M. Zhou
  • N. Panchuk-Voloshina

A Stable Nonfluorescent Derivative of Resorufin for the Fluorometric Determination of Trace Hydrogen Peroxide: Applications in Detecting the Activity of Phagocyte NADPH Oxidase and Other Oxidases

Anal Biochem . 1997 Nov 15 ; 253(2) 162 | DOI : https://doi.org/10.1006/abio.1997.2391

  • M. Zhou
  • et al

ΔΨm-Dependent and -independent production of reactive oxygen species by rat brain mitochondria

J Neurochem . 2001 Oct 01 ; 79(2) 266 | DOI : https://doi.org/10.1046/j.1471-4159.2001.00548.x

  • TV. Votyakova
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Fluorometric method for the enzymatic determination of cholesterol

J Biochem Biophys Methods . 1999 Jan 13 ; 38(1) 43 | DOI : https://doi.org/10.1016/S0165-022X(98)00036-0

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Hydrogen peroxide formation during iron deposition in horse spleen ferritin using O2 as an oxidant

Biochemistry . 2001 Feb 23 ; 40(11) 3340 | DOI : https://doi.org/10.1021/bi001981v

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  • et al

Quantitative relationship between inhibition of respiratory complexes and formation of reactive oxygen species in isolated nerve terminals

J Neurochem . 2003 Jan 01 ; 84(1) 112 | DOI : https://doi.org/10.1046/j.1471-4159.2003.01513.x

  • I. Sipos