Labeling-detection

AMCA-X, SE [6-((7-Amino-4-methylcoumarin-3-acetyl)amino)hexanoic acid, SE] - 10 mg

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  • Cat.Number : AS-81208
  • Availability :
    In production

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AMCA-X, SE is one of the most popular blue fluorescent tagging molecules. It is widely used to label antibodies, proteins and small drug molecules. AMCA-X succinimidyl ester contains a seven-atom aminohexanoyl spacer, the so-called ‘X’ spacer, between the fluorophore and the reactive group. It is well known that the spacer between the fluorophore and the reactive group usually separates the fluorophore from the biomolecule to which it is conjugated, potentially reducing the quenching that typically occurs upon conjugation. In some cases, the ‘X’ spacer allows the dye to be more available for recognition by secondary detection reagents. Abs/Em = 353/442 nm

Specifications

Chemistry
CAS registry number
  • 216309-02-3
Molecular Formula
  • C22H25N3O7
Molecular Mass/ Weight
  • 443.5
Properties
Absorbance (nm)
  • 353
Emission (nm)
  • 442
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥90%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMF or DMSO
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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References

7-Amino-4-methylcoumarin-3-acetic acid-conjugated streptavidin permits simultaneous flow cytometry analysis of either three cell surface antigens or one cell surface antigen as a function of RNA and DNA content

J Immunol Methods . 1990 Jan 01 ; 128(1) 39 | DOI : https://doi.org/10.1016/0022-1759(90)90461-4

  • JP. Aubry
  • et al

Increased resonance energy transfer between fluorophores bound to DNA in proximity to metallic silver particles

Anal Biochem . 2003 Mar 15 ; 315(2) 160 | DOI : https://doi.org/10.1016/S0003-2697(02)00710-8

  • J. Malicka
  • et al