Labeling-detection

HiLyte™ Fluor 405 succinimidyl ester (SE) - 1 mg

$150.00
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  • Cat.Number : AS-89317-1
  • Availability :
    In production
  • Shipping conditions : Ice fees will apply

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Quantity

HiLyte™ Fluor 405 is a blue fluorescent dye with Ex/Em = 404/428 nm, spectrally similar to Alexa Fluor™ 405 and DyLight Fluor™ 405 dyes. Conjugates of HiLyte™ Fluor 405 are well excited by the 407 nm spectral line of the Krypton laser and the 408 nm line of the violet laser diode. HiLyte™ Fluor 405 acid, SE is an amine reactive reagent for labeling peptides, proteins and antibodies. Extinction Coefficient (M-1cm-1): 35,000 Fluorescence quantum yield: 0.54

Specifications

Chemistry
Molecular Mass/ Weight
  • 785.6
Properties
Absorbance (nm)
  • 404
Emission (nm)
  • 428
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥90%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMF or DMSO: Prepare 10 mg/ml dye stock solution in high quality anhydrous DMSO. Completely dissolve all dye content by vortexing. Dye solution should be prepared fresh, immediately before an experiment. Long-term storage in solution form may reduce dye activity. Any solution containing the dye should be protected from light.
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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