Labeling-detection

HiLyte™ Fluor 488 succinimidyl ester (SE)

$158.00
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  • Cat.Number : AS-81161-1
  • Availability :
    In stock
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HiLyte™ Fluor 488 acid, SE is an excellent amine-reactive fluorescent labeling dye that generates the protein conjugates far superior to those of fluorescein derivatives such as FITC. Not only are HiLyte™ Fluor 488 conjugates significantly brighter than fluorescein conjugates, they are much more photostable. Additionally the fluorescence of HiLyte™ Fluor 488 fluorophore is independent of pH from 4 to 10. This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only above pH 10. Extinction Coefficient (M-1cm-1): 70,000; Fluorescence quantum yield: 0.91; Fluorescence Life Time (ns): 4.1

Specifications

Chemistry
Molecular Mass/ Weight
  • 583.6
Properties
Absorbance (nm)
  • 502
Emission (nm)
  • 527
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥80%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMF or DMSO: Prepare 10 mg/ml dye stock solution in high quality anhydrous DMSO. Completely dissolve all dye content by vortexing. Dye solution should be prepared fresh, immediately before an experiment. Long-term storage in solution form may reduce dye activity. Any solution containing the dye should be protected from light.
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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