IMG-EN-AS-72170-01.jpg
Assay-kits

SensoLyte® 520 Cathepsin D Assay Kit Fluorimetric - 1 kit

$582.00
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  • Cat.Number : AS-72170
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Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown. Cathepsin D is involved in the pathogenesis of several diseases such as breast cancer and Alzheimer disease.
The SensoLyte® 520 Cathepsin D Activity Assay Kit uses a QXL™ 520/HiLyte™ Fluor 488 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, which is optimal for cathepsin activity. The kit can be used to detect the activity of Cathepsin D enzyme in biological samples and purified enzyme preparations.

Specifications

Packaging
Kits components
  • Component A: QXL™ 520/HiLyte™ Fluor 488, Cathepsin D substrate, Ex/Em=490 nm/520 nm upon cleavage: 1 mM, 50 µL Component B: HiLyte™ Fluor 488, fluorescence reference standard, Ex/Em=490 nm/520 nm: 1 mM, 10 µL Component C: Cathepsin D, bovine spleen: 0.1 mg/mL, 10 µL Component D: Assay Buffer: 20 mL Component E: Pepstatin A: 100 µM, 20 µL Component F: DTT: 1 M, 100 µL
Chemistry
UniProt number
  • P07339
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 0.78 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

High-Fat Diet–Induced Adipocyte Cell Death Occurs Through a Cyclophilin D Intrinsic Signaling Pathway Independent of Adipose Tissue Inflammation

Diabetes . 2011 Jul 06 ; 60 2134 | DOI : 10.2337/db10-1411

  • D. Feng

Functional Heterogeneity of CD11c-positive Adipose Tissue Macrophages in Diet-induced Obese Mice

J Biol Chem . 2010 Mar 22 ; 285 15333 | DOI : 10.1074/jbc.M110.100263

  • P.P. Li

Bovine cathepsin D activity under high pressure

Food Chem . 2010 May 15 ; 120 474 | DOI : https://doi.org/10.1016/j.foodchem.2009.10.040

  • R. Buckow

Galloyl moieties enhance the dentin biomodification potential of plant-derived catechins

Acta Biomater . 2001 Apr 08 ; 10(7) 3288 | DOI : 10.1016/j.actbio.2014.03.036

  • C.M. Vidal

Type II alveolar cell MHCII improves respiratory viral disease outcomes while exhibiting limited antigen presentation

Nature Comm . 2021 Jun 28 ; 12 3993 | DOI : https://doi.org/10.1038/s41467-021-23619-6

  • SA. Toulmin
  • et al

Admission Value of Serum Cathepsin D Level Can be Useful for Predicting In-Hospital Mortality in Patients with NSTEMI

Acta Cardiol Sin. . 2017 Jul 01 ; 33(4) 393 | DOI : 10.6515/ACS20170129A

  • H. Kundi
  • et al

Mild MPP+ exposure impairs autophagic degradation through a novel lysosomal acidity-independent mechanism

J Neurochem . 2016 Jun 16 ; 139(2) 294 | DOI : https://doi.org/10.1111/jnc.13700

  • M. Miyara
  • et al

ADAM30 Downregulates APP-Linked Defects Through Cathepsin D Activation in Alzheimer's Disease

EBioMedicine . 2016 Jun 09 ; 9 278 | DOI : https://doi.org/10.1016/j.ebiom.2016.06.002

  • F. Letronne
  • et al

Chronic toxicity evaluation of the flame retardant tris (2-butoxyethyl) phosphate (TBOEP) using Daphnia magna transcriptomic response

Chemosphere . 2015 Aug 01 ; 132 159 | DOI : https://doi.org/10.1016/j.chemosphere.2015.03.028

  • M. Giraudo
  • et al

Elevated vasoinhibin derived from prolactin and cathepsin D activities in sera of patients with preeclampsia

Hypertension Res . 2015 Sep 17 ; 38 899 | DOI : https://doi.org/10.1038/hr.2015.99

  • R. Nakajima
  • et al