Peptides

Bacterial Sortase Substrate II , Dabcyl/Edans - 1 mg

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  • Cat.Number : AS-62230
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    In stock

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This peptide is a C-terminal surface sorting signal with a conserved LPXTG motif, labeled with the Dabcyl/Edans FRET pair. Sortase cleaves surface proteins at the LPXTG motif and catalyzes the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Sortases are a family of Gram-positive transpeptidases responsible for anchoring surface protein virulence factors to the peptidoglycan cell wall layer. Surface proteins of Staphylococcus aureus are anchored to the bacterial cell wall by a mechanism requiring this C-terminal sorting signal. Cell wall sorting is the covalent attachment of surface proteins to the peptidoglycan via a C-terminal sorting signal that contains a consensus LPXTG sequence. Cleavage of this FRET substrate by sortase reveals the fluorescent signal that may be measured to study sortase activity. Inhibition of the sortase activity is a potential way of treatment of the staphylococcal infection. The LPXTG motif is conserved in more than 100 surface proteins of Gram-positive pathogens.

Specifications

Chemistry
Sequence one letter code
  • Dabcyl-QALPETGEE-Edans
Sequence three letter code
  • Dabcyl-Gln-Ala-Leu-Pro-Glu-Thr-Gly-Glu-Glu-Edans
Molecular Mass/ Weight
  • 1472.7
Properties
Absorbance (nm)
  • 340
Emission (nm)
  • 490
Modification
Conjugation type
  • Double dyes
Modification Name
Conjugation
  • Conjugated
Quantity & Purity
Purity
  • Peak Area by HPLC ≥95%
Storage & stability
Form
  • Lyophilized
Storage Conditions
  • - 20 °C Protected from light
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • bacteria

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References

Flavonols inhibit sortases and sortase-mediated Staphylococcus aureus clumping to fibrinogen

Biol Pharm Bulletin . 2006 Aug 01 ; 29(8) 1751 | DOI : 10.1248/bpb.29.1751

  • S. Kang

Surface proteins of gram-positive bacteria and mechanisms of their targeting to the cell wall envelope

Mol. Biol. Rev. . 1999 Mar 01 ; 63(1) 174 | DOI : https://doi.org/10.1128/MMBR.63.1.174-229.1999

  • W. Navarre
  • O. Schneewind

Structure of sortase, the transpeptidase that anchors proteins to the cell wall of Staphylococcus aureus

PNAS . 2001 May 02 ; 98(11) 6056 | DOI : https://doi.org/10.1073/pnas.101064198

  • U. Ilangovan
  • et al

Purification and characterization of sortase, the transpeptidase that cleaves surface proteins of Staphylococcus aureus at the LPXTG motif

PNAS . 1999 Oct 26 ; 96(22) 12424 | DOI : https://doi.org/10.1073/pnas.96.22.12424

  • H. Ton-That
  • et al