IMG-EN-AS-85112-01.jpg
Labeling-detection

Collagen (Type IV), FAM conjugated - 1 mg

$461.00
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  • Cat.Number : AS-85112
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In this protease substrate, collagen is heavily labeled with a fluorescein derivative, resulting in almost total quenching of the conjugate's fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity. Collagen, Type IV should be particularly useful in the development of HTS assays for screening Gelatinase A (MMP-2) and Gelatinase A inhibitors, as well as for other gelatinases and collagenases that specifically degrade (Type IV) Collagen. Additionally, lightly fluorescein-labeled collagen may be useful for continuous assays monitored by fluorescence polarization.

Specifications

Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Color
Quantity & Purity
Concentration
  • 1 mg/ml
Storage & stability
Form
  • In solution
Resuspension condition
  • Assay buffer, pH>7
Storage Buffer
  • 0.01 M acetic acid
Storage Conditions
  • Store at -20°C and protect from light.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • human

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References

An improved assay method for fibroblast gelatinolytic enzyme

J Nihon Univ Sch Dent . 1997 Dec 01 ; 39(4) 182 | DOI : 10.2334/josnusd1959.39.182

  • K. Otsuka
  • et al

Enzymatic degradation of collagen-guided tissue regeneration membranes by periodontal bacteria.

Clin Oral Implants Res . 2003 May 20 ; 14(3) 263 | DOI : https://doi.org/10.1034/j.1600-0501.2003.140302.x

  • MN. Sela
  • et al

Fluorescence Polarization Assay and SDS-PAGE Confirms Matrilysin Degrades Fibronectin and Collagen IV whereas Gelatinase: A Degrades Collagen IV but not Fibronectin

Connect Tissue Res . 2001 Jan 01 ; 42(2) 149 | DOI : https://doi.org/10.3109/03008200109014256

  • PJ. Kraft
  • et al