Elastin, FITC Conjugated - 1 mg
- Cat.Number : AS-85113
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This substrate is soluble bovine neck ligament elastin that is heavily labeled with FITC such that the conjugate's fluorescence is quenched. Upon digestion by elastase or other proteases, the fluorescence is recovered. Digestion products from the elastin substrate have absorption maxima at ~490 nm and fluorescence emission maxima at ~520 nm. Because the assay is continuous, kinetics data can be obtained easily. The elastin derivative is also digested by proteases other than elastase.
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References
Determination of elastolytic activity with elastin-rhodamine
Anal Biochem . 1976 Aug 01 ; 74(2) 419 | DOI : https://doi.org/10.1016/0003-2697(76)90222-0
- PF. Huebner
- et al
Elastin degradation
1987 . 1987 Jan 01 ; 144 288 | DOI : https://doi.org/10.1016/0076-6879(87)44184-0
- MJ. Banda
- et al