SensoLyte® 520 MMP-12 Assay Kit Fluorimetric - 1 kit
- Cat.Number : AS-71157
- Manufacturer Ref. :
-
Availability :
In stock
- Shipping conditions : Ice fees will apply
Alternative choices
Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMP-12 (macrophage elastase) is expressed in activated macrophages and may play a role in smoke-induced emphysema.
SensoLyte® 520 MMP-12 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. This substrate showed excellent specificity to MMP-12 with minimal cross-reaction with MMP-1, 2, 7, 8, 9, 9, 13 and 14. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-12, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
Specifications
Packaging | |
Kits components |
|
---|---|
Chemistry | |
UniProt number |
|
Properties | |
Absorbance (nm) |
|
Emission (nm) |
|
Storage & stability | |
Storage Conditions |
|
Activity | |
Application | |
Biomarker Target | |
Detection Method | |
Detection Limit |
|
Research Area | |
Sub-category Research Area | |
Usage |
|
Downloads
You may also be interested in the following product(s)
Citations
The endogenous protease inhibitor TIMP-1 mediates protection and recovery from cutaneous photodamage
J Invest Dermatol. . 2012 Jun 21 ; 132(12) 2800 | DOI : 10.1038/jid.2012.204
- U. Yokose
Matrix metalloproteinase-12 (MMP-12) SNP affects MMP activity, lung macrophage infiltration and protects against emphysema in COPD
Thorax . 2011 Jul 05 ; 66(11) 970 | DOI : 10.1136/thx.2011.159087
- I. Haq
Modulation of the angiogenic phenotype of normal and systemic sclerosis endothelial cells by gain-loss of function of pentraxin 3 and matrix metalloproteinase 12
Arthritis Rheumatol . 2010 Aug 01 ; 62(8) 2488 | DOI : 10.1002/art.27522
- F. Margheri
Avenanthramide C as a novel candidate to alleviate osteoarthritic pathogenesis.
BMB Rep. . 2021 Oct 03 ; 54(10) 528 | DOI : 10.5483/BMBRep.2021.54.10.108
- TT. Tran
- et al