Assay-kits

SensoLyte® Plus 520 MMP-9 Assay Kit Fluorimetric and Enhanced Selectivity - 1 kit

$601.00
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  • Cat.Number : AS-72017
  • Availability :
    In production
  • Shipping conditions : Ice fees will apply

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The SensoLyte® Plus 520 MMP-9 Assay Kit is designed for specifically detecting MMP-9 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-9 monoclonal antibody is used in combination with a MMP fluorogenic substrate, 5-FAM/QXL™520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-9-induced cleavage of the FRET substrate.

Specifications

Packaging
Kits components
  • Component A: Microplate coated with monoclonal anti human MMP-9 antibody: 12 X 8 black strips Component B: MMP-9 standard, recombinant human proMMP-9: 10 µg/mL, 10 µL Component C: MMP dilution buffer: 5 mL Component D: 10 X Wash buffer: 50 mL Component E: APMA, 4-aminophenylmercuric acetate (Caution: Contain organic mercury. Dispose it according to your local regulations): 100 mM, 150 µL Component F: MMP-9 substrate, 5-FAM/QXL™520 FRET peptide Ex/Em=490 nm/520 nm upon cleavage: 50 µL Compo
Chemistry
UniProt number
  • P14780
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components, except Component B, at -20°C. Store Component B at -80°C. For convenience, Components D, G, H, I can be stored at RT.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 3 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

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Proteolytic activity in wound fluids and tissues derived from chronic venous leg ulcers

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Inhibition of MMP-3 or -9 suppresses lipopolysaccharide-induced expression of proinflammatory cytokines and iNOS in microglia

JNC . 2008 Jul 04 ; 106(2) 770 | DOI : https://doi.org/10.1111/j.1471-4159.2008.05430.x

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