IMG-EN-AS-85103-01.jpg
Labeling-detection

Elastin, FITC-Conjugated Water Insoluble - 1 mg

$247.00
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  • Cat.Number : AS-85103
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Besides the water-soluble elastin conjugate (Cat # AS-85113), this water-insoluble fluorescent conjugate can be used for fluorometric measurement of elastase activity. This product is insoluble bovine neck ligament elastin that is heavily labeled with FITC such that the conjugate's fluorescence is quenched. Upon digestion by elastase or other proteases, the fluorescence is recovered. Digestion products from the elastin substrate have absorption maxima at ~490 nm and fluorescence emission maxima at ~520 nm. This insoluble elastin derivative is more specific for elastase than AS-85113.

Specifications

Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Color
Quantity & Purity
Purity
  • Minimum Purity: 95%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • Assay buffer
Storage Conditions
  • Store at -20°C and protect from light and moisture.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • Bovine

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References

Determination of elastolytic activity with elastin-rhodamine. Anal Biochem

Anal Biochem . 1976 Aug 01 ; 74(2) 419 | DOI : https://doi.org/10.1016/0003-2697(76)90222-0

  • PF. Huebner
  • et al

Elastin degradation

1987 . 1987 Jan 01 ; 144 288 | DOI : https://doi.org/10.1016/0076-6879(87)44184-0

  • MJ. Banda
  • et al