Labeling-detection

HiLyte™ Fluor 594 hydrazide - 1 mg

$179.00
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  • Cat.Number : AS-81274
  • Availability :
    In production
  • Shipping conditions : Ice fees will apply

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Quantity

HiLyte™ Fluor 594, an alternative to Alexa Fluor™ 594 and DyLight Fluor™ 594, has spectral characteristics similar to those of Texas Red. The labeling performance and stability are better than those of Texas Red. It has high extinction coefficient (80,000 M-1cm-1), high fluorescence quantum yield (0.9) and low correction factor (0.17). HiLyte FluorTM 594 based Bioconjugates exhibit little spectral overlap with green-fluorescent conjugates, and can be efficiently excited by 568 nm line of Ar-Kr laser and by the 594 nm line of orange He-Ne laser. Extinction Coefficient (M-1cm-1): 80,000 Fluorescence quantum yield: 0.90 Fluorescence Life Time (ns): 4.2. HiLyte™ Fluor 594 hydrazide is a carbonyl-reactive fluorescent labeling dye. It can be used for labeling glycoproteins such as HRP.

Specifications

Chemistry
Molecular Mass/ Weight
  • 879.9
Properties
Absorbance (nm)
  • 593
Emission (nm)
  • 616
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥90%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMF or DMSO: Prepare 10 mg/ml dye stock solution in high quality anhydrous DMSO. Completely dissolve all dye content by vortexing. Dye solution should be prepared fresh, immediately before an experiment. Long-term storage in solution form may reduce dye activity. Any solution containing the dye should be protected from light.
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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