HIV Protease FRET Substrate I - 1 mg

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  • Cat.Number : AS-22992
  • Availability :
    In stock


DABCYL-GABA-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS is also called HIV protease substrate I in some literature. It is widely used for the continuous assay for HIV protease activity. The 11-kD protease (PR) encoded by the human immunodeficiency virus 1 (HIV-1) is essential for the correct processing of viral polyproteins and the maturation of infectious virus, and is therefore a target for the design of selective acquired immunodeficiency syndrome (AIDS) therapeutics. The FRET-based fluorogenic substrate is derived from a natural processing site for HIV-1 PR. Incubation of recombinant HIV-1 PR with the fluorogenic substrate resulted in specific cleavage at the Tyr-Pro bond and a time-dependent increase in fluorescence intensity that is linearly related to the extent of substrate hydrolysis. The fluorescence quantum yields of the HIV-1 PR substrate in the FRET assay increased by 40.0- and 34.4-fold, respectively, per mole of substrate cleaved. Because of its simplicity and precision in the determination of reaction rates required for kinetic analysis, this substrate offers many advantages over the commonly used HPLC or electrophoresis-based assays for peptide substrate hydrolysis by retroviral PRs. Abs/Em = 340nm/490nm.


Sequence one letter code
Sequence three letter code
  • DABCYL-GABA-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS
CAS registry number
  • 127134-13-8
Molecular Formula
  • C73H97N17O18S
Molecular Mass/ Weight
  • 1532.8
Absorbance (nm)
  • 340
Emission (nm)
  • 490
Conjugation type
  • Double dyes
Modification Name
  • Conjugated
Quantity & Purity
  • Peak Area by HPLC ≥95%
Storage & stability
  • Lyophilized
Storage Conditions
  • - 20 °C Protected from light
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
  • Research use
Source / Species
  • HIV

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Sensitive, hydrosoluble, macromolecular fluorogenic substrates for human immunodeficiency virus 1 proteinase

Biochem J . 1993 May 01 ; 291 (Pt3) 869 | DOI : 10.1042/bj2910869.

  • F. Anjuere

Fluorescence-based continuous assay for the aspartyl protease of human immunodeficiency virus-1

FEBS Lett . 1990 Jan 22 ; 262 119 | DOI : 10.1016/0014-5793(90)80168-i

  • KF. Geohegan
  • et al