Protein

Human MMP-10 - 100 µL

  • Cat.Number : AS-72067
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    In stock
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Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. MMP-10 (stromelysin 2) is involved in several pathological conditions, such as cancer, arthritis and wound healing. MMP-10 is secreted as zymogen with a prodomain, a catalytic domain, a hinge region, and a hemopexin-like domain. It can activate other MMPs such as MMP-1, MMP-8 and degrade a variety of substrates, including gelatin, collagens III, IV and V, fibronectin, aggrecan

This recombinant human MMP-10 was expressed as a pro-enzyme enzyme from its DNA sequence7 transfected into a mouse myeloma cell line, NS0. The apparent Mr on SDS-PAGE is 58-kDa. Incubation with 1 mM APMA at 37°C for 1-2 hours will activate pro-MMP-10. Its activity can be measured by FRET peptides

Specifications

Chemistry
UniProt number
  • P09238
Molecular Mass/ Weight
  • 58 kDa
Modification
Conjugation
  • Unconjugated
Quantity & Purity
Concentration
  • 10 µg/ml
Storage & stability
Form
  • In solution
Storage Buffer
  • 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM CaCl2, 1 mg/mL BSA. Endotoxin level is less than 1.0 EU per µg of enzyme determined by LAL method.
Storage Conditions
  • Store at -80 ºC. Avoid repeated freeze-thaw cycles.
Activity
Application
Biomarker Target
Unit definition
  • One unit of protease hydrolyzes 1 picomole of Mca-Arg-Pro-Lys-Pro-Val-GluNva-Trp-Arg-Lys(Dnp)-NH2 per minute at pH 7.5 at 25°C
Specificity
  • Human MMP-10
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host
Source / Species
  • human

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