Protein

Human Renin, recombinant - 5 µg

$112.00
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  • Cat.Number : AS-72041
  • Availability :
    In stock
  • Shipping conditions : Ice fees will apply

Quantity

Renin, a highly specific aspartyl protease, cleaves angiotensinogen, produced in the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood vessels, leading to increased blood pressure. It also increases the secretion of ADH and aldosterone, and stimulates the hypothalamus to activate the thirst reflex. Since an overactive renin-angiotensin system leads to hypertension, renin is proposed as a therapeutic target for this disease

The recombinant human pro-renin was expressed in HEK cells and converted to the active renin. After affinity chromatography, the active enzyme has a purity of >99% by SDS-PAGE. The molecular mass of active human renin is approximately 40 kDa.
The activity of enzyme can be measured in FRET-based assays

Specifications

Chemistry
UniProt number
  • P00797
Molecular Mass/ Weight
  • 40 kDa
Modification
Conjugation
  • Unconjugated
Quantity & Purity
Concentration
  • 50 µg/ml
Purification Method
  • Affinity purified
Storage & stability
Form
  • In solution
Storage Buffer
  • 50 mM MOPS, pH 7.0 with BSA and NaN3.
Storage Conditions
  • Store at -80°C. Avoid repeated freeze-thaw cycles.
Activity
Application
Biomarker Target
Unit definition
  • One unit of protease hydrolyzes 1 picomole of FRET peptide (ArgGlu(EDANS)-Ile-His-Pro-Phe-His-Leu-Val-Il2-His-Thr-Lys(Dabcyl)-Arg) per minute
Specificity
  • Human renin
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host
Source / Species
  • human

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