Assay-kits

SensoLyte® 390 Generic MMP Activity Kit Fluorimetric - 1 kit

$393.00
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  • Cat.Number : AS-72202
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    In stock
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Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMPs play important roles in tumor development and invasion, as well in many diseases.
The SensoLyte® 390 Generic MMP Assay Kit is optimized to detect the activity of a variety of MMPs, including MMP-1, 2, 7, 8, 9, 13, 14, 15, 16, and 24. This kit can be used for detecting generic MMP activity in biological samples or for high throughput screening of MMP inducers and inhibitors using purified MMPs. This kit provides an Mca/Dnp fluorescence resonance energy transfer (FRET) peptide as a MMP substrate. In the intact FRET peptide, the fluorescence of Mca is quenched by Dnp. Upon cleavage into two separate fragments by MMPs, the fluorescence of Mca is recovered, and can be monitored at excitation/emission wavelengths = 330 nm/390 nm. The assays are performed in a convenient 96-well microplate format.

Specifications

Packaging
Kits components
  • Component A: Mca/Dnp, MMP substrate, Ex/Em=330 nm /390 nm upon cleavage: 50 ml Component B: Mca fluorescence reference standard, Ex/Em=330nm /390 nm: 1 mM, 10 mL Component C: APMA, 4-aminophenylmercuric acetate: 1M, 20 mL Component D: Assay buffer: 20 mL Component E: Stop Solution: 10 mL
Properties
Absorbance (nm)
  • 330
Emission (nm)
  • 390
Storage & stability
Storage Conditions
  • Store all components at -20°C. Protect Components A and B from light. Components D and E can be stored at 4°C for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • nanogram
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Sphingosine-1-Phosphate Protects Endothelial Glycocalyx by Inhibiting Syndecan-1 Shedding

Am J Physiol Heart Circ Physiol . 2013 Nov 27 ; 306(3) H363 | DOI : 10.1152/ajpheart.00687.2013

  • Y. Zeng
  • et al