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Assay-kits

SensoLyte® 440 Cathepsin S Assay Kit Fluorimetric - 1 kit

$527.00
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  • Cat.Number : AS-72100
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Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin S is a cysteine proteinase involved in the pathogenesis of autoimmune diseases, atherosclerosis, cancer, obesity and related diseases.
The SensoLyte® 440 Cathepsin S Assay Kit uses a fluorogenic peptide for measurement of enzyme activity. This peptide releases the AMC (7-amino-4-methylcoumarin) fluorophore upon cathepsin S cleavage, and which can be detected with excitation at 354 nm and emission at 442 nm.

Specifications

Packaging
Kits components
  • Component A: Cathepsin S substrate, Ex/Em=354 nm/442 nm upon cleavage: 4 mM, 50 µL Component B: AMC, fluorescence reference standard, Ex/Em=354 nm/442 nm: 4 mM, 10 µL Component C: Cathepsin S, human spleen: 0.1 mg/mL, 10 µL Component D: Assay Buffer: 20 mL Component E: Cathepsin S Inhibitor: 1 mM, 10 µL Component F: DTT: 1 M, 100 µL
Chemistry
UniProt number
  • P25774
Properties
Absorbance (nm)
  • 354
Emission (nm)
  • 442
Storage & stability
Storage Conditions
  • Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 1 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host

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Citations

Ultralarge Virtual Screening Identifies SARS-CoV-2 Main Protease Inhibitors with Broad-Spectrum Activity against Coronaviruses

J. Am. Chem. Soc. . 2022 Feb 10 ; 144(7) 2905 | DOI : https://doi.org/10.1021/jacs.1c08402

  • A. Luttens

Differential effects of specific cathepsin S inhibition in biocompartments from patients with primary Sjögren syndrome

Arthritis Res Ther . 2019 Jul 18 ; 21 175 | DOI : https://doi.org/10.1186/s13075-019-1955-2

  • P. Hargreaves
  • et al