Assay-kits

SensoLyte® 490 MMP-13 Assay Kit Fluorimetric - 1 kit

$663.00
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  • Cat.Number : AS-71135
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Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMP-13 (collagenase-3, rat collagenase) is involved in quite a few diseases such as arthritis and asthma.
The SensoLyte® 490 MMP-13 Assay Kit is a complete assay system designed to continuously analyze MMP-13 activities or to screen MMP-13 inhibitors by using a fluorogenic MMP-13 substrate.
Members of the MMP family have poor substrate sequence specificity, making it difficult to use a peptide substrate alone to differentiate the activity of a particular MMP from other MMPs. If several MMPs are coexisting in your samples and you would like to specifically measure MMP-13 activity, please choose the SensoLyte® Plus MMP-13 Assay Kit, Cat# AS-72019.

Specifications

Packaging
Kits components
  • Component A: MMP-13 substrate EDANS/DabcylPlus™ FRET peptide, Ex/Em=340 nm/490 nm upon cleavage: 270 µL Component B: EDANS, fluorescence reference standard, Ex/Em=340 nm/490 nm: 1 mM DMSO solution, 10 µL Component C: APMA, 4-aminophenylmercuric acetate (Caution: Toxic! Handle with care): 1 M, 100 µL Component D: Assay buffer: 60 mL Component E: Stop solution: 30 mL
Chemistry
UniProt number
  • P45452
Properties
Absorbance (nm)
  • 340
Emission (nm)
  • 490
Storage & stability
Storage Conditions
  • Store all components at -20°C. Protect Components A and B from light. Components D and E can be stored at 4°C for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

The cross-talk between transforming growth factor-beta1 and ultrasound stimulation during mechanotransduction of rat tenocytes

Connect Tissue Res . 2010 Nov 30 ; 52(4) 313 | DOI : 10.3109/03008207.2010.525673

  • Y.H. Chao
  • et al