IMG-EN-AS-71134-01.jpg
Assay-kits

SensoLyte® 490 MMP-9 Assay Kit Fluorimetric - 1 kit

$804.00
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  • Cat.Number : AS-71134
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Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMP-9 (gelatinase-B, collagenase-IV) is involved in quite a few diseases such as a cancer, angiogenesis, alopecia and metastasis.
The SensoLyte® 490 MMP-9 Assay Kit is a complete assay system designed to continuously analyze MMP activity or to screen for MMP-9 inhibitors using a fluorogenic MMP-9 substrate. The assays are performed in a convenient 96-well or 384-well microplate format. The kit provides speed, convenience and accuracy in MMP-9 analysis.
Members of the MMP family have poor substrate sequence specificity, making it difficult to use a peptide substrate alone to differentiate the activity of a particular MMP from other MMPs. If several MMPs are coexisting in your samples and you would like to specifically measure MMP-9 activity, please choose the SensoLyte® Plus MMP-9 Assay Kit, Cat# AS-72017.

Specifications

Packaging
Kits components
  • Component A: MMP-9 substrate Ex/Em=340 nm/490 nm 270 µL Component B: EDANS, fluorescence reference standard Ex/Em=340 nm/490 nm: 1 mM DMSO solution, 10 µL Component C: APMA, 4-aminophenylmercuric acetate (Caution: Toxic! Handle with care): 1 M, 100 µL Component D: Assay buffer: 60 mL Component E: Stop solution: 30 mL
Chemistry
UniProt number
  • P14780
Properties
Absorbance (nm)
  • 340
Emission (nm)
  • 490
Storage & stability
Storage Conditions
  • Store all components at -20°C. Protect components A and B from light. Components D and E can be stored at 4 °C for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Progesterone attenuates hemorrhagic transformation after delayed tPA treatment in an experimental model of stroke in rats: involvement of the VEGF–MMP pathway

J Cereb Blood Flow Metab . 2013 Sep 18 ; 34(1) 72 | DOI : 10.1038/jcbfm.2013.163

  • S. Won

Syndecan-1 enhances the endometrial cancer invasion by modulating matrix metalloproteinase-9 expression through nuclear factor kappaB

Gynecol Oncol . 2009 Jun 17 ; 114(3) 509 | DOI : 10.1016/j.ygyno.2009.05.027

  • J.H. Oh

Quercetin inhibition of tumor invasion via suppressing PKCδ/ERK/AP-1-dependent matrix metalloproteinase-9 activation in breast carcinoma

Carcinogenesis. . 2008 Jul 14 ; 29(9) 1807 | DOI : 10.1093/carcin/bgn162

  • C. Lin

ERK1/2 activation induced by inflammatory cytokines compromises effective host tissue integration of engineered cartilage

Tissue Eng Part A . 2008 Jan 01 ; 15(10) 2825 | DOI : 10.1089/ten.TEA.2008.0663

  • F. Djouad

The inhibitory effects of silver diamine fluoride at different concentrations on matrix metalloproteinases

Dent Mater. . 2012 May 12 ; 28(8) 903 | DOI : 10.1016/j.dental.2012.04.011

  • M.L. Mei

Alzheimer's disease protease-containing plasma extracellular vesicles transfer to the hippocampus via the choroid plexus

EBioMedicine . 2022 Mar 01 ; 77 103903 | DOI : https://doi.org/10.1016/j.ebiom.2022.103903

  • J-H. Lee

Doxycycline prevents blood–brain barrier dysfunction and microvascular hyperpermeability after traumatic brain injury.

Sci Rep . 2022 Mar 30 ; 12(1) 5415 | DOI : https://doi.org/10.1038/s41598-022-09394-4

  • B. Robinson
  • et al

Phenethyl isothiocyanate inhibits the migration and invasion of colon cancer SW480 cells via the inhibition of matrix metalloproteinase-9

Int J Clin Exp Med . 2016 Jan 01 ; 9(2) 2423

  • H. Li
  • et al