IMG-EN-AS-72226-01.jpg
Assay-kits

SensoLyte® 520 ADAM10 Activity Assay Kit Fluorimetric - 1 kit

$631.00
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  • Cat.Number : AS-72226
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ADAM10 has been shown to be an effective α-secretase in vitro and in vivo. This enzyme acts as a sheddase to cleave cell surface proteins involved in neuropathology, inflammatory response and tumor progression.
The SensoLyte® 520 ADAM10 Activity Assay Kit is an optimized assay that can be used to detect the ADAM 10 activity. The unique FRET substrate contained in the kit was designed to reduce the cross reactivity with ADAM17 (also called tumor necrosis factor-α-converting enzyme, TACE). When active ADAM10 cleaves the FRET substrate, it results in an increase of 5-FAM fluorescence monitored at excitation/emission = 490/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.25 ng/mL active ADAM10.

Specifications

Packaging
Kits components
  • Component A: 5-FAM /QXL™ 520 ADAM10 substrate Ex/Em=490 nm/520 nm upon cleavage: 1 mM, 50µL Component B: 5-FAM fluorescence reference standard, Ex/Em=490 nm/520 nm: 1 mM, 12 µL Component C: Human recombinant ADAM10: 0.1 mg/mL, 10 µL Component D: Assay Buffer: 25 mL Component E: Inhibitor: 1 mM, 10µL
Chemistry
UniProt number
  • O14672
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 0.25 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host

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Citations

ADAM10 and BACE1 are localized to synaptic vesicles

J Neurochem . 2015 Sep 17 ; 135(3) 606-15 | DOI : 10.1111/jnc.13287

  • L.J. Lundgren

The effect of citalopram treatment on amyloid-β precursor protein processing and oxidative stress in human hNSC-derived neurons

Translational Psychiatry . 2022 Jun 18 ; 12 285 | DOI : https://doi.org/10.1038/s41398-022-02050-5

  • RJ. Elsworthy
  • et al

Nogo-66 promotes β-amyloid protein secretion via NgR/ROCK-dependent BACE1 activation

Mol Med Rep . 2021 Mar 01 ; 23(3) 188 | DOI : 10.3892/mmr.2021.11827

  • Q-Q. Xie
  • et al

Autophagy-dependent increased ADAM10 mature protein induced by TFEB overexpression is mediated through PPARα

Mol Neurobiol . 2021 May 01 ; 58(5) 2269 | DOI : 10.1007/s12035-020-02230-8

  • H. Wang
  • et al

Dysregulated APP expression and α-secretase processing of APP is involved in manganese-induced cognitive impairment

Ecotoxicol Environment Safety . 2021 Sep 01 ; 220 112365 | DOI : https://doi.org/10.1016/j.ecoenv.2021.112365

  • Y. Yang
  • et al

Klotho regulation by albuminuria dependent on ATF3 and endoplasmatic reticulum stress

The FACEB J. . 2020 Feb 11 ; 34(2) 2087 | DOI : https://doi.org/10.1096/fj.201900893R

  • V. Delitsikou
  • et al

Substrate-Specific Activation of α-Secretase by 7-Deoxy-Trans-Dihydronarciclasine Increases Non-Amyloidogenic Processing of β-Amyloid Protein Precursor

Molecules . 2020 Feb 03 ; 25(3) 646 | DOI : https://doi.org/10.3390/molecules25030646

  • Y S Chun
  • et al

Shear stress activates ADAM10 sheddase to regulate Notch1 via the Piezo1 force sensor in endothelial cells

eLife. . 2020 Jun 02 ; 9 e50684 | DOI : 10.7554/eLife.50684.

  • V. Caolo
  • et al

TNFα promotes mucosal wound repair through enhanced Platelet Activating Factor Receptor signaling in the epithelium

Mucosal Immunol . 2019 Jul 01 ; 12(4) 909 | DOI : 10.1038/s41385-019-0150-8

  • D. Birkl
  • et al

ADAM10 Cell Surface Expression but Not Activity Is Critical for Staphylococcus aureus α-Hemolysin-Mediated Activation of the NLRP3 Inflammasome in Human Monocytes

Toxins . 2016 Mar 30 ; 8(4) 95 | DOI : https://doi.org/10.3390/toxins8040095

  • E A D Ezekwe
  • et al

Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells

J Immunol . 2015 Jun 15 ; 195 (2) 736 | DOI : https://doi.org/10.4049/jimmunol.1402643

  • A. Zingoni
  • et al