IMG-EN-AS-72171-01.jpg
Assay-kits

SensoLyte® 520 Cathepsin K Assay Kit Fluorimetric - 1 kit

$607.00
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  • Cat.Number : AS-72171
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Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin K is the lysosomal cysteine protease involved in bone remodeling and resorption. It has potential as a drug target in autoimmune diseases and osteoporosis.
The SensoLyte® 520 Cathepsin K Activity Assay Kit uses a QXL™ 520/HiLyte™ Fluor 488 FRET peptide substrate for the measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490/520 nm. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, which is optimal for cathepsin activity. The kit can be used to detect the activity of Cathepsin K enzyme in biological samples and purified enzyme preparations.

Specifications

Packaging
Kits components
  • Component A: QXL™ 520/HiLyte™ Fluor 488, Cathepsin K substrate, Ex/Em=490 nm/520 nm upon cleavage: 2 mM, 50 µL Component B: HiLyte™ Fluor 488, fluorescence reference standard, Ex/Em=490 nm/520 nm: 1 mM, 10 µL Component C: Procathepsin K, human recombinant: 0.15 mg/mL, 10 µL Component D: Assay Buffer: 20 mL Component E: Cathepsin K activation buffer: 100 µL Component F: Cathepsin K Inhibito:r 100 µM, 10 µL Component G: DTT: 1 M, 200 µL
Chemistry
UniProt number
  • P43235
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host

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Citations

Optimizing resin-dentin bond stability using a bioactive adhesive with concomitant antibacterial properties and anti-proteolytic activities

Acta Biomater . 2018 Jul 15 ; 75 171 | DOI : 10.1016/j.actbio.2018.06.008

  • Y-P Gou
  • et al

Artemisia annua extract prevents ovariectomy-induced bone loss by blocking receptor activator of nuclear factor kappa-B ligand-induced differentiation of osteoclasts

Scientific Reports . 2017 Dec 11 ; 7 17332  | DOI : https://doi.org/10.1038/s41598-017-17427-6

  • S. Lee
  • et al