Assay-kits

SensoLyte® pNPP Alkaline Phosphatase Assay Kit Colorimetric - 1 kit

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  • Cat.Number : AS-72146
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The change of alkaline phosphatase activity is involved in a variety of physiological and pathological events, such as bone development, bone-related diseases, gestation-related diseases and inflammatory bowel diseases. Alkaline phosphatase is also a popular enzyme conjugated with secondary antibody for ELISA. p-Nitrophenyl phosphate (pNPP) is proven to be an effective chromogenic substrate for alkaline phosphate.
This SensoLyte® kit can be used to detect alkaline phosphatase activity in biological samples and alkaline phosphatase-based ELISA. The kit contains: pNPP chromogenic liquid substrate (absorption maximum = 405 nm upon dephosphorylation), Assay buffer, Stop solution, Alkaline phosphatase standard, “mix and read” assay protocol that is compatible with high throughput screening.

Specifications

Packaging
Kits components
  • Component A: pNPP, colorimetric alkaline phosphatase substrate: 25 mL Component B: 10X Assay buffer: 50 mL Component C: Stop solution: 25 mL Component D: Triton-X-100: 500 µL Component E: Alkaline Phosphatase Standard, Calf Intestine: 10 µg/mL, 50 µL
Properties
Absorbance (nm)
  • 405
Storage & stability
Storage Conditions
  • Protect component A from light. Store all components at 4°C. Store Component B and Component E at -20°C
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 0.2 ng/ml
Research Area
Usage
  • Research use

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Citations

Amelioration of the fitness costs of antibiotic resistance due to reduced outer membrane permeability by upregulation of alternative porins

MolBioEvol . 2015 Sep 09 ; 15 | DOI : 10.1093/molbev/msv195

  • M. Knopp

Cabozantinib Inhibits Prostate Cancer Growth and Prevents Tumor-Induced Bone Lesions

Clin Cancer Res . 2013 Oct 04 ; 20 617 | DOI : 10.1158/1078-0432.CCR-13-0839

  • J. Dai

The effect of epigallocatechin-3-gallate (EGCG) on human alveolar bone cells both in vitro and in vivo

Archives Oral Biol . 2014 Mar 02 ; 59 539 | DOI : 10.1016/j.archoralbio.2014.02.011

  • Y.J. Mah

Biocompatibility of silver nanoparticles and silver ions in primary human mesenchymal stem cells and osteoblasts

Acta Biomat . 2013 Oct 03 ; 10 439 | DOI : https://doi.org/10.1016/j.actbio.2013.09.037

  • L. Pauksch

Liquid fructose downregulates Sirt1 expression and activity and impairs the oxidation of fatty acids in rat and human liver cells

Biochim Biophys Acta -Mol Cell Biol Lipids . 2014 Jan 13 ; 1841(4) 514 | DOI : 10.1016/j.bbalip.2014.01.002

  • A. Rebollo

The effect of a bioactive collagen membrane releasing PDGF or GDF-5 on bone regeneration

Biomat . 2013 Dec 30 ; 35 2446 | DOI : 10.1016/j.biomaterials.2013.12.006

  • S. Yamano

Facile surface functionalization with glycosaminoglycans by direct coating with mussel adhesive protein

Tissue Eng Part C Meth . 2011 Nov 08 ; 18 71 | DOI : 10.1089/ten.TEC.2011.0384

  • B. Choi

The establishment of a primary culture system of proximal tubule segments using specific markers from normal mouse kidneys

Intl J Mol Sci . 2012 Jan 23 ; 13 5098 | DOI : 10.3390/ijms13045098

  • M. Kamiyama

Gelatin-layered and multi-sized porous β-tricalcium phosphate for tissue engineering scaffold

Nanoscale Res Lett . 2012 Jan 17 ; 7 78 | DOI : 10.1186/1556-276X-7-78Open Access

  • S-M. Kim

Characterization of ocular surface epithelial and progenitor cell markers in human adipose stromal cells derived from lipoaspirates

Invest Ophthalmol Vis Sci . 2012 Jan 31 ; 53 513-520 | DOI : 10.1167/iovs.11-7550

  • E. Martinez-Conesa

Heat treatment of Na2O-CaO-P2O5-SiO2 bioactive glasses: Densification processes and postsintering bioactivity

J Biomed Mat Res . 2011 Nov 02 ; 100A 305 | DOI : 10.1002/jbm.a.33276

  • A. Sola

Biomimetic interpenetrating polymer network hydrogels based on methacrylated alginate and collagen for 3D pre-osteoblast spreading and osteogenic differentiation

Soft Matter . 2012 Jan 12 ; 8 2398 | DOI : 10.1039/C2SM06869A

  • J. Sun

Highly efficient antiviral and antibacterial activities of solid-state cuprous compounds

J Haz Mat . 2012 Aug 06 ; 265 235-236 | DOI : 10.1002/jbm.a.33276

  • K. Sunada

The external mechanical environment can override the influence of local substrate in determining stem cell fate

J Biomechanics . 2012 Aug 25 ; 45 2483 | DOI : 10.1016/j.jbiomech.2012.07.024

  • M. Thorpe

Hydrostatic pressure acts to stabilize a chondrogenic phenotype in porcine joint tissue derived stem cells

Euro Cells and Mat . 2012 Feb 23 ; 23 121 | DOI : 10.22203/ecm.v023a09

  • T. Vinardell

A comparison of human smooth muscle and mesenchymal stem cells as potential cell sources for tissue-engineered vascular patches

Tissue Eng Part A . 2012 Jan 26 ; 18 986-98 | DOI : 10.1089/ten.TEA.2011.0172

  • C. Williams

Effects of cryopreservation with a newly-developed magnetic field programmed freezer on periodontal ligament cells and pulp tissues

Cryobiol . 2011 Jan 03 ; 62(3) 181-187 | DOI : 10.1016/j.cryobiol.2011.03.001.

  • S. Abedini

Highly bioactive 8 nm hydrothermal TiO2 nanotubes elicit enhanced bone cell response

Adv Eng Mat . 2011 Jan 17 ; 13 B88 | DOI : https://doi.org/10.1002/adem.201080103

  • K. Brammer

Comparative cell behavior on carbon-coated TiO2 nanotube surfaces for osteoblasts vs. osteo-progenitor cells

Acta Biomaterialia . 2011 Jun 01 ; 7 2697 | DOI : https://doi.org/10.1016/j.actbio.2011.02.039

  • K. Brammer

Zirconium oxide nanotube surface prompts increased osteoblast functionality and mineralization

Mat Sci and Eng . 2011 Dec 01 ; 31 1716 | DOI : https://doi.org/10.1016/j.msec.2011.07.016

  • C. Frandsen

Protein composition of microparticles shed from human placenta during placental perfusion: Potential role in angiogenesis and fibrinolysis in preeclampsia

Placenta . 2010 Nov 11 ; 32 63 | DOI : 10.1016/j.placenta.2010.10.011

  • S. Guller

Quantitative proteomic analysis of dexamethasone-induced effects on osteoblast differentiation, proliferation, and apoptosis in MC3T3-E1 cells using SILAC.

Osteo Inter . 2011 Jul 01 ; 22(7) 2175 | DOI : 10.1007/s00198-010-1434-8

  • D. Hong

Affinity-based growth factor delivery using biodegradable, photocrosslinked heparinalginate hydrogels.

J of Cont Rel . 2011 Jul 02 ; 154 258 | DOI : 10.1016/j.jconrel.2011.06.027

  • O. Jeon

The role of cell surface markers and enamel matrix derivatives on human periodontal ligament mesenchymal progenitor responses in vitro.

Biomat . 2011 Jul 23 ; 32 7375 | DOI : 10.1016/j.biomaterials.2011.06.043

  • P. Kemoun

Recognition of β-Strand motifs by RseB is required for E activity in escherichia coli.

J Bacteriol . 2011 Sep 09 ; 193 6179-6186 | DOI : 10.1128/JB.05657-11

  • A. Kulp

Effect of low-magnitude, high-frequency vibration on osteogenic differentiation of rat mesenchymal stromal cells.

J Orthop Res. . 2011 Feb 22 ; 29 1075 | DOI : 10.1002/jor.21334

  • E. Lau

Role of mitogen-activated protein kinase in osteoblast differentiation

J Ortho Res . 2010 Aug 30 ; 29 204 | DOI : 10.1002/jor.21222

  • F. Lin

Contribution of bacterial outer membrane vesicles to innate bacterial defense

Biomedcentral . 2011 Dec 01 ; 11 258 | DOI : 10.1186/1471-2180-11-258

  • A. Manning

Polycaprolactone fibres as a potential delivery system for collagen to support bone regeneration

Curr Drug Deliv . 2011 Jul 01 ; 8 448 | DOI : 10.2174/156720111795767951

  • S. McNeil

Mesenchymal stem cell derived hematopoietic cells are permissive to HIV-1 infection

Retrovirol . 2011 Jan 12 ; 8(1) 3 | DOI : 10.1186/1742-4690-8-3

  • T. Nazari-Shafti

Schlafen-3 decreases cancer stem cell marker expression and autocrine/juxtacrine signaling in FOLFOX-resistant colon cancer cells

AJP-GI . 2011 May 19 ; 301(2) G347 | DOI : 10.1152/ajpgi.00403

  • P-S. Oh

Effect of various concentrations of antibiotics on osteogenic cell viability and activity

J Orthopaedic Res . 2011 Feb 24 ; 29(7) 1070 | DOI : 10.1002/jor.21343.

  • C. Rathbone

Processing/structure/property relationship of multi-scaled PCL and PCL–HA composite scaffolds prepared via gas foaming and NaCl reverse templating

Biotech and Bioengin . 2010 Dec 14 ; 108 963 | DOI : 10.1002/bit.23018

  • A. Salerno

Cell invasion of poultry-associated Salmonella enterica serovar Enteritidis isolates is associated with pathogenicity, motility and proteins secreted by the type III secretion system.

Microbiol . 2011 Feb 03 ; 157(pt5) 1428 | DOI : 10.1099/mic.0.044461-0.

  • D. Shah

Osteoconductivity and growth factor production by MG63 osteoblastic cells on bioglass-coated orthopedic implants

Biotech and Bioengin . 2011 Feb 01 ; 108 454 | DOI : 10.1002/bit.22955

  • F. Tan

The effect of scaffold architecture on odontogenic differentiation of human dental pulp stem cells

Biomaterials . 2011 Jun 12 ; 32(31) 7822 | DOI : 10.1016/j.biomaterials.2011.04.034

  • J. Wang

Bioactive collagen membrane as a carrier for sustained release of PDGF

JTisSciEng . 2011 Jan 01 ; 2 4 | DOI : 10.4172/2157-7552.1000110

  • S. Yamano

Localized colonic stem cell transplantation enhances tissue regeneration in murine colitis

JCellMolMed . 2012 Aug 01 ; | DOI : 10.1111/j.1582-4934.2011.01485.x

  • Q. Zhou

Propensity to high-fat diet-induced obesity in rats is associated with changes in the gut microbiota and gut inflammation

AmJPhysiolGastrointestLiverPhysiol . 2010 May 27 ; 299 G440 | DOI : 10.1152/ajpgi.00098.2010

  • C. Barbier de La Serre

Cell behavior on extracellular matrix mimic materials based on mussel adhesive protein fused with functional peptides

Biomaterials . 2010 Sep 15 ; 31 8980 | DOI : 10.1016/j.biomaterials.2010.08.027

  • B. Choi

Persistent high glucose concentrations alter the regenerative potential of mesenchymal stem cells

StemCellsDev . 2010 Sep 11 ; 19 1875 | DOI : 10.1089/scd.2010.0009

  • C. Cramer

Requirement of TGFβ signaling for SMO-mediated carcinogenesis

JBiolChem . 2010 Sep 21 ; 285(47) 36570-6 | DOI : 10.1074/jbc.C110.164442

  • Q. Fan

PTHrP 1-141 and 1-86 increase in vitro bone formation

JSurgRes . 2010 Mar 16 ; 162 e9 | DOI : 10.1016/j.jss.2010.02.023

  • B. Hildreth

Morphological and proteomic analysis of early stage of osteoblast differentiation in osteoblastic progenitor cells

ExperimentalRes . 2010 May 17 ; 316 2291 | DOI : 10.1016/j.yexcr.2010.05.011

  • D. Hong

Response of human embryonic stem cells derived mesenchymal stem cells to osteogenic factors and architectures of materials during in vitro osteogenesis

TissueEngineerPartA . 2010 Aug 17 ; 16(11) 3507-14 | DOI : 10.1089/ten.TEA.2010.0097

  • J. Hu

The Wnt/beta-catenin pathway regulates growth and maintenance of colonospheres

MolCancer . 2010 Aug 06 ; 6 212 | DOI : 10.1186/1476-4598-9-212.

  • S. Kanwar

Bone morphogenetic protein 6 drives both osteogenesis and chondrogenesis in murine adipose-derived mesenchymal cells depending on culture conditions

BiochemBiophysResCommun . 2010 Sep 09 ; 401(1) 20-5 | DOI : 10.1016/j.bbrc.2010.08.135

  • C. Kemmis

MT1-MMP controls human mesenchymal stem cell trafficking and differentiation

Blood . 2009 Nov 09 ; 115(2) 221-9 | DOI : 10.1182/blood-2009-06-228494

  • C. Lu

Effect of nanoparticulate bioactive glass particles on bioactivity and cytocompatibility of poly(3-hydroxybutyrate) composites.

JRSocInterface . 2009 Jul 29 ; 7(44) 453-65 | DOI : 10.1098/rsif.2009.0255

  • S. Misra

JAK2 V617F uses distinct signalling pathways to induce cell proliferation and neutrophil activation

BritishjournalofHaematology . 2009 Jun 10 ; 150 334 | DOI : https://doi.org/10.1111/j.1365-2141.2010.08249.x

  • S. Oku

Biomineralized porous composite scaffolds prepared by chemical synthesis for bone tissue regeneration

ActaBiomater . 2010 Apr 24 ; 10 4090-4099 | DOI : https://doi.org/10.1016/j.actbio.2010.04.018

  • M.G. Raucci

Characterization of hTERT-immortalized osteoblast cell lines generated from wild-type and connexin43-null mouse calvaria

AmJPhysiolCellPhysiol . 2010 Aug 04 ; 299 C994 | DOI : https://doi.org/10.1152/ajpcell.00544.2009

  • M.M. Thi

The odontogenic differentiation of human dental pulp stem cells on nanofibrous poly(L-lactic acid) scaffolds in vitro and in vivo.

ActaBiomater . 2010 Apr 18 ; 10 3856 | DOI : 10.1016/j.actbio.2010.04.009

  • J. Wang

A comparison of bioreactors for culture of fetal mesenchymal stem cells for bone tissue engineering

Biomaterials . 2010 Aug 24 ; 31 8684 | DOI : 10.1016/j.biomaterials.2010.07.097

  • Z. Zhang

Opiates inhibit paclitaxel uptake by P-glycoprotein in preparations of human placental inside-out vesicles

BiochemPharm . 2009 Jul 08 ; 78 1272 | DOI : 10.1016/j.bcp.2009.07.002

  • S. Hemauer

Replicative senescence of vascular smooth muscle cells enhances the calcification through initiating the osteoblastic transition

AmJPhysiolHeartCircPhysiol . 2009 Sep 11 ; 297 H1673-H1684 | DOI : 10.1152/ajpheart.00455.2009

  • R. Kurimoto-Nakano

Modular peptides promote human mesenchymal stem cell differentiation on biomaterial surfaces

ActaBiomater . 2009 Aug 06 ; 6 21 | DOI : 10.1016/j.actbio.2009.08.003

  • J. Lee

Inhibitor of DASH proteases affects expression of adhesion molecules in osteoclasts and reduces myeloma growth and bone disease

BritishJofHematology . 2009 Apr 08 ; 145 775 | DOI : 10.1111/j.1365-2141.2009.07696.x

  • A. Pennisi

OTU domain-containing ubiquitin aldehyde-binding protein 1

JBiolChem . 2009 Apr 21 ; 284(24) 16135 | DOI : 10.1074/jbc.M109.007484

  • V. Stanisic

Superior osteogenic capacity for bone tissue engineering of fetal compared with perinatal and adult mesenchymal stem cells

StemCells . 2009 Jan 01 ; 27 126 | DOI : 10.1634/stemcells.2008-0456

  • Z. Zhang

CD133+CD44+ population efficiently enriches colon cancer initiating cells

AnnSurgOncol . 2008 Jul 29 ; 15 2927 | DOI : 10.1245/s10434-008-0074-0

  • N. Haraguchi

Regulating the age-related oxidative damage, mitochondrial integrity, and antioxidative enzyme activity in fischer 344 rats by supplementation of the antioxidant epigallocatechin-3-gallate

RejuvRes . 2008 Jun 01 ; 11 649 | DOI : 10.1089/rej.2007.0645

  • Q. Meng

Engineering a disulfide bond to stabilize the calcium binding loop of activated protein C eliminates its anticoagulant but not protective signaling properties

JBiolChem . 2007 Jan 25 ; 282(12) 9251 | DOI : 10.1074/jbc.M610547200

  • J. Bae

Identification of a specific exosite on activated protein C for interaction with protease-activated receptor 1

JBiolChem . 2007 Aug 31 ; 282 25493 | DOI : 10.1074/jbc.M702131200

  • L. Yang

Deletion of the P2Y2 receptor aggravates internal elastic lamina calcification in chronic kidney disease mice through upregulation of alkaline phosphatase and lipocalin-2

FASEB J. . 2023 Jan 01 ; 37(1) e22701 | DOI : https://doi.org/10.1096/fj.202201044R

  • B. Opdebeeck
  • et al

Maternal high-fat diet promotes calcified atherosclerotic plaque formation in adult offspring by enhancing transformation of VSMCs to osteochondrocytic-like phenotype.

Heliyon . 2022 Sep 15 ; 8(9) e10644 | DOI : https://doi.org/10.1016/j.heliyon.2022.e10644

  • D. Miyawaki
  • et al

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BioMed Res Internat . 2022 Jul 14 ; 2022 9818299 | DOI : https://doi.org/10.1155/2022/9818299

  • Y-M. Song
  • et al

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PloS One . 2022 Mar 23 ; 17(3) e0265532 | DOI : https://doi.org/10.1371/journal.pone.0265532

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  • et al

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BMC Complem Med Therap . 2021 Jan 25 ; 21 44 | DOI : https://doi.org/10.1186/s12906-021-03212-6

  • Y-J. Son
  • et al

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Nutrients . 2021 Mar 10 ; 13(4) 1251 | DOI : 10.3390/nu13041251

  • TR. Price
  • et al

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PNAS . 2020 Feb 04 ; 117 (7) 3568 | DOI : https://doi.org/10.1073/pnas.1908830117

  • X. Li
  • et al