SensoLyte® FDP Alkaline Phosphatase Assay Kit Fluorimetric - 1 kit

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  • Cat.Number : AS-71109
  • Availability :
    In stock
  • Shipping conditions : Ice fees will apply


Alkaline phosphatase is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics. Conjugates of calf intestinal alkaline phosphatase are extensively used as secondary detection reagents in ELISAs, immunohistochemical techniques and Northern, Southern and Western blot analyses. SensoLyte® FDP Alkaline Phosphatase Assay Kit uses highly purified FDP to quantify alkaline phosphatase activity in solutions, in cell extracts, in live cells as well as on solid surfaces (such as PVDF membranes). The kit contains: FDP phosphatase substrate (Ex/Em=485±20/528±20 nm upon dephosphorylation), Calibration standard, Reaction buffer, Stop buffer, An optimized ‘mix and read’ assay protocol that is compatible with HTS liquid handling instruments


Kits components
  • Component A: FDP, fluorogenic alkaline phosphatase substrate: 1 vial Component B: 2X Assay buffer: 30 mL Component C: Stop solution: 30 mL Component D: 10X Lysis buffer: 50 mL Component E: Triton X-100: 500 µL Component F: DMSO: 500 µL Component G Alkaline Phosphatase Standard, Calf Intestine: 10 µg/mL, 50 µL, sterile
Absorbance (nm)
  • 485
Emission (nm)
  • 528
Storage & stability
Storage Conditions
  • Store component A at -20°C. Store all other components at 4°C.
Biomarker Target
Detection Method
Detection Limit
  • 5 pg/ml
Research Area
Sub-category Research Area
  • Research use

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