Assay-kits

SensoLyte® FDP Alkaline Phosphatase Assay Kit Fluorimetric - 1 kit

$300.00
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  • Cat.Number : AS-71109
  • Availability :
    In stock
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Alkaline phosphatase is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics. Conjugates of calf intestinal alkaline phosphatase are extensively used as secondary detection reagents in ELISAs, immunohistochemical techniques and Northern, Southern and Western blot analyses. SensoLyte® FDP Alkaline Phosphatase Assay Kit uses highly purified FDP to quantify alkaline phosphatase activity in solutions, in cell extracts, in live cells as well as on solid surfaces (such as PVDF membranes). The kit contains: FDP phosphatase substrate (Ex/Em=485±20/528±20 nm upon dephosphorylation), Calibration standard, Reaction buffer, Stop buffer, An optimized ‘mix and read’ assay protocol that is compatible with HTS liquid handling instruments

Specifications

Packaging
Kits components
  • Component A: FDP, fluorogenic alkaline phosphatase substrate: 1 vial Component B: 2X Assay buffer: 30 mL Component C: Stop solution: 30 mL Component D: 10X Lysis buffer: 50 mL Component E: Triton X-100: 500 µL Component F: DMSO: 500 µL Component G Alkaline Phosphatase Standard, Calf Intestine: 10 µg/mL, 50 µL, sterile
Properties
Absorbance (nm)
  • 485
Emission (nm)
  • 528
Storage & stability
Storage Conditions
  • Store Component A at –20°C. Store other kit components at 4°C.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 5 pg/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Effects of pseudowollastonite (CaSiO3) bioceramic on in vitro activity of human mesenchymal stem cells

Biomaterials . 2010 Aug 02 ; 31 7653 | DOI : 10.1016/j.biomaterials.2010.06.043

  • N. Zhang
  • et al

Nerve growth factor promotes differentiation of odontoblast-like cells

J Cell Biochem . 2009 Mar 01 ; 106 539 | DOI : 10.1002/jcb.22006

  • S. Arany
  • et al

Hedgehog acyltransferase as a target in pancreatic ductal adenocarcinoma

Oncogene . 2014 Jan 27 ; 34 263 | DOI : 10.1038/onc.2013.575

  • E. Petrova
  • et al