SensoLyte® 520 Cathepsin L Assay Kit Fluorimetric - 1 kit
- Cat.Number : AS-72218-
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Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin L, a lysosomal endopeptidase, is a member of the papain-like family of cysteine proteinases. It is involved in the promotion of tumor cell invasion, antigen processing and turnover of intracellular and secreted proteins.
The SensoLyte® 520 Cathepsin L Activity Assay Kit uses a QXL™ 520/HiLyte™ Fluor 488 labeled FRET peptide substrate for measuring enzyme activity. The long wavelength FRET substrate is designed based on a sequence surrounding the cleavage site of Cathepsin L. The QXL™ 520/HiLyte™ Fluor 488 pair is used for optimal quenching of the intact substrate. When active Cathepsin L cleaves the FRET substrate, it results in an increase of HiLyte Fluor™ 488 fluorescence and is monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, the optimal pH for Cathepsin L activity.
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Citations
Dual Inhibition of HIV-1 and Cathepsin L Proteases by Sarcandra glabra
Molecules . 2022 Sep 01 ; 27(17) 5552 | DOI : 10.3390/molecules27175552
- B. Pan
- et al
Novel dithiocarbamates selectively inhibit 3CL protease of SARS-CoV-2 and other coronaviruses
Eurn J Med Chem . 2023 Mar 15 ; 250 115186 | DOI : https://doi.org/10.1016/j.ejmech.2023.115186
- L. Brier
- et al