Assay-kits

SensoLyte® 520 Enterokinase Assay Kit Fluorimetric - 1 kit

$552.00
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  • Cat.Number : AS-72209
  • Availability :
    In production
  • Shipping conditions : Ice fees will apply

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Enterokinase or enteropeptidase is a heterodimeric serine protease produced by cells in the duodenal wall. It converts trypsinogen to trypsin, indirectly activating a number of pancreatic digestive enzymes. Enterokinase is a key enzyme for intestinal digestion of proteins. Its deficiency may cause severe protein malabsorption leading to impaired development and growth. Enterokinase also plays an important role in acute experimental and clinical pancreatitis. The high degree of specificity exhibited by enteropeptidase for cleaving affinity or other tags from recombinant proteins makes it an ideal tool for use in biochemical applications.
The SensoLyte® 520 Enterokinase Assay Kit employs a novel internally quenched 5-FAM/QXL™ FRET substrate for the detection of enterokinase activity. The enzyme cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence which can be monitored at excitation /emission= 490/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assays are performed in a convenient 96-well microplate format.

Specifications

Packaging
Kits components
  • Component A: 5-FAM/QXL™-520 Enterokinase substrate, Ex/Em=490/520 nm upon cleavage: 2 mM, 50 µL Component B: 5-FAM, fluorescence reference standard, Ex/Em=490/520 nm: 2 mM, 10 µL Component C: Recombinant Bovine Enterokinase: 40 µL Component D: 2X Assay Buffer: 25 mL Component E: Inhibitor E-64: 50 mM, 20 µL
Chemistry
UniProt number
  • P98073
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 1.25 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host

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