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Assay-kits

SensoLyte® 520 Meprin a Activity Assay Kit Fluorimetric - 1 kit

$669.00
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  • Cat.Number : AS-72253
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Meprin α is a zinc endopeptidase of the astacin family and the metzincin superfamily. Meprin α can cleave extracellular matrix proteins (pro-collagen I, fibronectin, osteopontin , SPARC), growth factors (VEGF-A, CTGF) and proteinases (MMP-1). Evidence has suggested that Meprin α is associated with cancer proliferation, cancer migration and invasion.
The FRET substrate included in SensoLyte® 520 Meprin α Activity Assay Kit was designed to detect Meprin α activity and reduce the cross reactivity with Meprin β, ADAM10, β-secretase, BACE-2, ECEs and TACE. This assay kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. Active Meprin α cleaves the FRET substrate, resulting in an increase of 5-FAM fluorescence, monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.1 ng/mL active Meprin α.

Specifications

Packaging
Kits components
  • Component A: 5-FAM /QXL® 520 Meprin a substrate Ex/Em=490 nm/520 nm upon cleavage 0.2 mM, 50 µL Component B: 5-FAM fluorescence reference standard, Ex/Em=490 nm/520 nm 0.2 mM, 15 µL Component C: Human recombinant Meprin a: 0.05 mg/mL, 20 µL Component D: Meprin a activation buffer: 100 µL Component E: Meprin a stop solution: 20 µL Component F: 2X Assay Buffer: 25 mL Component G: Inhibitor: 50 µM, 200 µL
Chemistry
UniProt number
  • Q16819
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store component C at -80°C. Store all other components at -20°C. Protect components A and B from light and moisture. Component F can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 0.1 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host

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Citations

Mechanisms of GLP-1 receptor–independent renoprotective effects of the dipeptidyl peptidase type 4 inhibitor linagliptin in GLP-1 receptor knockout mice with 5/6 nephrectomy

Kidney International . 2019 Jun 01 ; 95(6) 1373 | DOI : https://doi.org/10.1016/j.kint.2019.01.010

  • A. Hasan
  • et al