Assay-kits

SensoLyte® 520 MMP-14 Assay Kit Fluorimetric - 1 kit

$480.00
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  • Cat.Number : AS-72025
  • Availability :
    In stock
  • Shipping conditions : Ice fees will apply

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Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. MMP-14 (MT1-MMP) is the membrane bound MMP. When expressed in cells, the catalytic and the hemopexin domains of MMP-14 become exposed on cell surfaces. This enzyme plays an important role in tumor invasion. SensoLyte® 520 MMP-14 Assay Kit uses a 5-FAM (fluorophore) and QXL™520 (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon the cleavage of the FRET peptide by MMP-14, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL™520 based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.

Specifications

Packaging
Kits components
  • Component A: MMP-14 substrate 5-FAM/QXL™520 FRET peptide Ex/Em=490nm/520nm upon cleavage: 60 µL Component B: 5-FAM-Pro-Leu-OH, fluorescence reference standard, Ex/Em=490 nm/520 nm: 1 mM, 10 µL Component C: APMA, 4-aminophenylmercuric acetate (Caution: Organic mercury. Handle with care! Do not dispose into sink!): 1 M, 20 µL Component D: Assay buffer: 20 mL Component E: Stop solution: 10 mL
Chemistry
UniProt number
  • P50281
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all components at -20°C. Protect Components A and B from light. Components D and E can be stored at 4°C for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 2 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Fluid shear stress and sphingosine 1-phosphate activate calpain to promote membrane type 1 matrix metalloproteinase

J Biol Chem . 2014 Oct 14 ; 286 42017 | DOI : 10.1074/jbc.M111.290841

  • H. Kang
  • et al

Graded activation of the MEK1/MT1-MMP axis determines renal epithelial cell tumor phenotype

Carcinogenesis . 2011 Sep 28 ; 32 1806 | DOI : 10.1093/carcin/bgr216

  • R. Mahimkar
  • et al

LOX-1-MT1-MMP axis is crucial for RhoA and Rac1 activation induced by oxidized low-density lipoprotein in endothelial cells

Cardiovasc Res . 2009 Jun 01 ; 84 127 | DOI : 10.1093/cvr/cvp17710.1093/cvr/cvp177

  • K. Sugimoto
  • et al