Assay-kits

SensoLyte® 520 MMP Profiling Kit Fluorimetric - 1 kit

$733.00
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  • Cat.Number : AS-71136
  • Availability :
    In stock
  • Shipping conditions : Ice fees will apply

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Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. They are involved in diseases such as cancer, arthritis, asthma, angiogenesis, alopecia and metastasis. They are also identified as drug-screening targets. SensoLyte® 520 MMP Profiling Kit contains a series of well-designed FRET peptide substrates for use as fluorogenic indicators to assay MMP protease activities. It provides the best solution for profiling MMPs or MMP inhibitors.

Specifications

Packaging
Kits components
  • Component A: Microplate pre-coated with 16 different MMP substrates. Ex/Em=490 nm/520 nm upon cleavage: 2 identical 96-well plates Component B: 5-FAM-Pro-Leu-OH, fluorescence reference standard Ex/Em=490 nm/520 nm: 1 mM, 10 µL Component C: APMA, 4-aminophenylmercuric acetate (Caution: Toxic! Handle with care): 1 M, 100 µL Component D: Assay buffer: 40 mL Component E: Stop solution: 20 mL
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all components at -20°C. Protect components A and B from light. Components D and E can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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