Assay-kits

SensoLyte® 520 MMP Substrate Sampler Kit Fluorimetric - 1 kit

$846.00
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  • Cat.Number : AS-71170
  • Availability :
    In stock
  • Shipping conditions : Ice fees will apply

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Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. They are involved in diseases such as cancer, arthritis, asthma, angiogenesis, alopecia and metastasis. They are also identified as drug-screening targets. SensoLyte® 520 MMP Substrate Sampler Kit contains a series of FRET peptide substrates for use as fluorogenic indicators to assay MMP protease activities. It provides the best solution for identifying a suitable fluorogenic substrate for a particular MMP assay. All the MMP substrates in the kit are also available in bulk packages.

Specifications

Packaging
Kits components
  • Component A: 16 different MMP substrates. Ex/Em=490 nm/520 nm upon cleavage: 100 µM, 150 µL each Component B: 5-FAM-Pro-Leu-OH, fluorescence reference standard Ex/Em=490 nm/520 nm: 1 mM, 10 µL Component C: APMA, 4-aminophenylmercuric acetate: 1 M, 100 µL Component D: Assay buffer: 50 mL Component E: Stop solution: 30 mL
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all components at -20°C. Protect components A and B from light. Components D and E can be stored at 4 °C for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

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J Biol Chem . 2006 Apr 28 ; 281(26) 17670 | DOI : 10.1074/jbc.M602487200

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A Barcode-Free Combinatorial Screening Platform for Matrix Metalloproteinase Screening.

Anal. Chem . 2015 Jan 01 ; 87(3) 1950 | DOI : https://doi.org/10.1021/ac504330x

  • T. Rane
  • et al

PI3K, Erk Signaling in BMP7-Induced Epithelial-Mesenchymal Transition (EMT) of PC-3 Prostate Cancer Cells in 2- and 3-Dimensional Cultures

Hormones and Cancer . 2011 Sep 27 ; 2 298 | DOI : https://doi.org/10.1007/s12672-011-0084-4

  • M. Lim
  • et al