Assay-kits

SensoLyte® ABD-F Thiol Quantitation Assay Kit “Fluorimetric” - 1 kit

$356.00
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  • Cat.Number : AS-72137
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    In stock
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Thiols, compounds that contain sulfhydryl group, are powerful reducing agents capable of acting as antioxidants, enzyme cofactors, and neuromodulators. The SensoLyte® ABD-F Thiol Quantitation Assay Kit provides a convenient, sensitive fluorescent assay for measurement of thiol concentrations. 4-fluoro-7-aminosulfonylbenzofurazan (ABD-F), the reagent provided in the kit, generates yellow-green fluorescence upon reaction with thiols. Fluorescence can be monitored at excitation/emission=389 nm/513 nm.

Specifications

Packaging
Kits components
  • Component A: Thiol Detection Reagent: 500 µL Component B Reduced Glutathione (GSH) Standard: 10 mM, 100 µL Component C: Assay Buffer: 100 mL
Properties
Absorbance (nm)
  • 389
Emission (nm)
  • 513
Storage & stability
Storage Conditions
  • Store all kit components at -20°C. Protect Component A from light and moisture. Component C can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 0.25 uM
Research Area
Sub-category Research Area
Usage
  • Research use

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