IMG-EN-AS-71116-01.jpg
Assay-kits

SensoLyte® AFC Caspase Profiling Kit Fluorimetric - 1 kit

$846.00
Check your price
  • Cat.Number : AS-71116
  • Availability :
    In stock
  • Shipping conditions : Ice fees will apply

Alternative choices

Quantity

Add to wishlist Bulk Quote

Caspases play important roles in apoptosis and cell signaling. They are also identified as drug-screening targets. AFC-based substrates yield blue fluorescence upon protease cleavage. They are widely used to monitor caspase activity.
The SensoLyte® Caspase Profiling Kit contains a series of AFC-based peptide substrates (Ex/Em=380 nm/500 nm) as fluorogenic indicators for assaying caspase protease activities. The kit contains a well-designed plate in which a series of AFC-based caspase substrates are coated with both positive and negative controls. It provides the best solution for profiling caspases or caspase inhibitors.

Specifications

Packaging
Kits components
  • Component A: Caspase microplate: pre-coated with 8 different caspase substrates Ex/Em=380 nm/500 nm upon cleavage: 2 96-well plates (8 x 12 strips) Component B: AFC, fluorescence reference standard Ex/Em=380 nm/500 nm: 10 mM, 20 µL Component C: Assay buffer: 40 mL Component D: DTT: 1 M, 1 mL Component E: 10X Lysis Buffer: 20 mL
Properties
Absorbance (nm)
  • 380
Emission (nm)
  • 500
Storage & stability
Storage Conditions
  • Store components A, B, and D at -20°C. Components C and E can be stored at 4°C for convenience. Component A and B protect from light and moisture.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

Downloads

You may also be interested in the following product(s)

IMG-EN-AS-61375-01.jpg

p53 (17-26) - 1 mg

Cat.Number : AS-61375
$95.00 Excl. Tax
IMG-EN-AS-62206-01.jpg

Proapoptotic Peptide, (klaklak)2, 5-FAM-labeled - 1 mg

Cat.Number : AS-62206
$296.00 Excl. Tax
IMG-EN-AS-71141-01.jpg

SensoLyte® Homogeneous Rh110 Caspase-3/7 Assay Kit Fluorimetric - 1 kit

Cat.Number : AS-71141
$382.00 Excl. Tax

Citations

Regulation of apoptosis and innate immune stimuli in inflammation-induced preterm labor.

J Immunol . 2013 Oct 25 ; 191(11) 5702 | DOI : 10.4049/jimmunol.1301604

  • M.K. Jaiswal

Endoplasmic reticulum stress induces a caspase-dependent N-terminal cleavage of RBX1 protein in B cells.

J Biol Chem . 2012 Jul 20 ; 387(37) 31223 | DOI : 10.1074/jbc.M111.314583

  • S. Shteingart