SensoLyte® Cell Cytotoxicity Assay Kit Fluorimetric - 1 kit
- Cat.Number : AS-71302
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The damage of cell membrane leads to the release of cytoplasmic enzymes. The measurement of released cytoplasmic lactate dehydrogenase (LDH) is a well-accepted assay to estimate cell membrane integrity and quantify cytotoxicity. The SensoLyte® Cell Cytotoxicity Assay Kit uses resazurin as a sensitive fluorogenic indicator (Ex/Em=560 nm/590 nm upon conversion) to measure LDH activity. The assay can be performed in a mixed population of damaged and viable cells, but it only measures the LDH released from damaged cells. The cytoplasmic LDH in living cells produces little signals under assay condition. There is no need for extra steps to separate living cells and supernatant. The fluorescent signal is proportional to the number of damaged cells (up to 2.5X104 cell, r2>0.95) with the detection limit reaching 100 dead cells. The kit is suitable for high throughput screening of cytotoxicity of a variety of compounds. 384-well or 1536-well format can be used with minor modifications.
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Citations
The mRNA-destabilizing protein tristetraprolin is suppressed in many cancers, altering tumorigenic phenotypes and patient prognosis
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Epidermal Growth Factor-Like Growth Factors Prevent Apoptosis of Alcohol-Exposed Human Placental Cytotrophoblast Cells
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Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor.
Development. . 2006 Jan 11 ; 133(4) 751 | DOI : 10.1242/dev.02237
- D.R. Armant
An experimental study of rabbit conjunctival epithelial toxicity using co-treatment with Mitomycin-C and a histone deacetylase inhibitor
Arch Pharmacal Res . 2010 Aug 28 ; 33 1261 | DOI : https://doi.org/10.1007/s12272-010-0817-x
- T.H. Kim
References
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Assessment of a simple, non-toxic Alamar blue cell survival assay to monitor tomato cell viability
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A new nondestructive cytometric assay based on resazurin metabolism and an organ culture model for the assessment of corneal viability
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- S. Perrot
- et al