SensoLyte® Cell Viability and Proliferation Assay Kit Fluorimetric, Large size - 1 kit
- Cat.Number : AS-71301
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Lactate dehydrogenase (LDH) exists ubiquitously in the cytoplasm of living mammalian cells and the measurement of cytoplasmic LDH activity is a well-accepted assay to quantify living cell numbers and indicate cell viablity. The DHL™ Cell Viability and Proliferation Assay Kit provides researchers with a convenient one-solution and one-step assay to count living cells in a culture and continuously monitor cell proliferation over time by measuring cytoplasmic LDH activity. In this kit, resazurin is used as a sensitive fluorogenic indicator. Resazurin is be converted to the strongly fluorescent resorufin (Ex/Em=560nm/590 nm) by cytoplasmic LDH. The kit can detect as few as 48 living cells with a linear range up to 5X104 cells (r2=0.99). This kit can also be used for high throughput screening of cell proliferation or cytotoxicity effect of a variety of compounds. 384-well or 1536-well format can be used with minor modifications. Kit Size: 10,000 assays
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Blood . 2010 Nov 09 ; 117(3) 920 | DOI : 10.1182/blood-2010-05-282921
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Cell Commun Signal . 2011 May 02 ; 9 10 | DOI : 10.1186/1478-811X-9-10
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