Assay-kits

SensoLyte® FDG ß-Galactosidase Assay Kit Fluorimetric - 1 kit

$300.00
Check your price
  • Cat.Number : AS-72133
  • Availability :
    In production
  • Shipping conditions : Ice fees will apply

Select specification

Quantity

β-galactosidase, encoded by the lacZ gene in E. coli, is widely used as a reporter enzyme to study gene expression, protein-protein interaction and normalization of transfection efficiency in mammalian cells. The SensoLyte® FDG β-Galactosidase Assay Kit provides a sensitive fluorogenic substrate for detecting β-galactosidase activity in tranfected cells. In the presence of β-galactosidase, the colorless fluorescein di-β-D galactopyranoside (FDG) substrate is hydrolyzed into a fluorescein that can be detected at Ex/Em = 490/520 nm.

Specifications

Packaging
Kits components
  • Component A: ß-galactosidase substrate: 10 mM, 250 µL Component B: Fluorescein, reference standard: 5 mM, 25 µL Component C: ß-galactosidase enzyme: 0.1 mg/mL, 20 µL Component D: Assay buffer: 100 mL Component E: DTT: 1 M, 4.5 mL Component F: Triton X-100: 200 L Component G: Stop solution: 30 mL
Chemistry
UniProt number
  • P16278
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store components at -20°C. Keep components A and B away from light. Components F and G can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 100 pg/ml
Research Area
Sub-category Research Area
Usage
  • Research use

You may also be interested in the following product(s)