Assay-kits

SensoLyte® MUG ß-Galactosidase Assay Kit Fluorimetric - 1 kit

$315.00
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  • Cat.Number : AS-72132
  • Availability :
    In stock
  • Shipping conditions : Ice fees will apply

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β-galactosidase, encoded by the lacZ gene in E. coli, is widely used as a reporter enzyme to study gene expression, protein-protein interaction and normalization of transfection efficiency in mammalian cells. The SensoLyte® MUG β-Galactosidase Assay Kit provides a highly sensitive fluorogenic substrate for detection of β-galactosidase activity in tranfected cells. In the presence of β-galactosidase, the colorless 4-methylumbelliferyl β-D-galactopyranoside (MUG) substrate is hydrolyzed into a product that has bright blue fluorescence.

Specifications

Packaging
Kits components
  • Component A: ß-galactosidase substrate: 20 mM, 250 µL Component B: 4MU, reference standard: 10 mM, 50 µL Component C: ß-galactosidase enzyme: 0.1 mg/mL, 20 µL Component D: Assay buffer: 100 mL Component E: DTT 1 M, 4.5 mL Component F: Triton X-100: 200 µL Component G: Stop solution: 30 mL
Chemistry
UniProt number
  • P16278
Properties
Absorbance (nm)
  • 360
Emission (nm)
  • 460
Storage & stability
Storage Conditions
  • Store all components at -20°C. Components D, F and G can be stored at room temperature for convenience. Protect components A and B from light.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 150 femtogram/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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