SensoLyte® FDP Protein Phosphatase Assay Kit Fluorimetric - 1 kit

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  • Cat.Number : AS-71100
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Protein phosphatases have received great attention as drug-screening targets. FDP is a highly sensitive fluorogenic substrate for most phosphatases, i.e., alkaline phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The phosphatase-induced hydrolysis of FDP yields an intense fluorescent product that has excitation wavelength around 490 nm, and emission maximum around 520 nm. The fluorescence of FDP hydrolyzed product is strengthened when pH > 7.0. For some protein phosphatase requiring slightly acidic buffer for reaction, a pH-adjusting stop solution should be added before reading the fluorescence signal. The kit can be used for characterizing kinetics of enzyme reaction and high throughput screening of protein phosphatase inducers and inhibitors.


Kits components
  • Component A: FDP, Ex/Em= 485±20/528±20 nm upon phosphate group removal: 1 vial Component B: Assay buffer, pH 6.5: 60 ml Component C: 10X Lysis buffer: 50 ml Component D: Triton X-100: 500 µL Component E: Stop solution: 30 ml Component F: 1 M DTT: 100 µl Component G: DMSO: 500 µl
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store components A and F at -20°C. Component B, C, D, E and G can be stored at room temperature for convenience.
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
  • Research use

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