Assay-kits

SensoLyte® FDP Protein Phosphatase Assay Kit Fluorimetric - 1 kit

$300.00
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  • Cat.Number : AS-71100
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Protein phosphatases have received great attention as drug-screening targets. FDP is a highly sensitive fluorogenic substrate for most phosphatases, i.e., alkaline phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The phosphatase-induced hydrolysis of FDP yields an intense fluorescent product that has excitation wavelength around 490 nm, and emission maximum around 520 nm. The fluorescence of FDP hydrolyzed product is strengthened when pH > 7.0. For some protein phosphatase requiring slightly acidic buffer for reaction, a pH-adjusting stop solution should be added before reading the fluorescence signal. The kit can be used for characterizing kinetics of enzyme reaction and high throughput screening of protein phosphatase inducers and inhibitors.

Specifications

Packaging
Kits components
  • Component A: FDP, Ex/Em= 485±20/528±20 nm upon phosphate group removal: 1 vial Component B: Assay buffer, pH 6.5: 60 ml Component C: 10X Lysis buffer: 50 ml Component D: Triton X-100: 500 µL Component E: Stop solution: 30 ml Component F: 1 M DTT: 100 µl Component G: DMSO: 500 µl
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C. Components B, C, D, E and G can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Neuroprotective copper bis(thiosemicarbazonato) complexes promote neurite elongation.

PLoS One . 2014 Feb 28 ; 9(2) e90070 | DOI : 10.1371/journal.pone.0090070

  • L. Bica
  • et al

Sulfhydration of p66Shc at cysteine59 mediates the anti-oxidant effect of Hydrogen Sulfide

Antioxid Redox Signal . 2014 Dec 20 ; 21(18) 2531 | DOI : 10.1089/ars.2013.5604

  • Z.Z. Xie
  • et al

The Bxb1 gp47 recombination directionality factor is required not only for prophage excision, but also for phage DNA replication

Gene . 2012 Mar 01 ; 495(1) 42 | DOI : 10.1016/j.gene.2011.12.003

  • A. Savinov
  • et al

A role for NANOG in G1 to S transition in human embryonic stem cells through direct binding of CDK6 and CDC25A

J Cell Biol . 2009 Jan 12 ; 184(1) 67 | DOI : 10.1083/jcb.200801009

  • X. Zhang
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The cytoplasmic tail of CD45 is released from activated phagocytes and can act as an inhibitory messenger for T cells

Blood . 2008 Aug 15 ; 112(4) 1240 | DOI : 10.1182/blood-2008-02-138131

  • S. Kirchberger
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