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Assay-kits

SensoLyte® pNPP Protein Phosphatase Assay Kit Colorimetric - 1 kit

$324.00
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  • Cat.Number : AS-71105
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Protein phosphatases have received great attentions as drug-screening targets. p-Nitrophenyl phosphate (pNPP) is proven to be an effective chromogenic substrate for protein tyrosine phosphatases and serine/threonine phosphatases. SensoLyte® pNPP Protein Phosphatase Assay Kit uses pNNP to quantify protein phosphatase activities. The kit can be used for characterizing kinetics of enzyme reaction and high throughput screening of protein phosphatase inhibitors. It has high sensitivity and wide linear range (The detection limit is generally 3 ng or below). The kit contains: - pNNP chromogenic substrate - Protein phosphatase inhibitor - Assay buffer - A 'mix and read' assay protocol that is compatible with HTS liquid handling instruments

Specifications

Packaging
Kits components
  • Component A: pNPP, absorbance=405 nm upon phosphate group removal: 1 vial Component B: Assay buffer: 60 mL Component C: 10X Lysis buffer: 50 mL Component D: Triton X-100: 500 µL Component E: Stop solution: 30 mL Component F: 1 M DTT: 100 µL
Properties
Absorbance (nm)
  • 405
Storage & stability
Storage Conditions
  • Store components A and F at -20°C. Component B, C, D, and E can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 100 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Liquid fructose downregulates Sirt1 expression and activity and impairs the oxidation of fatty acids in rat and human liver cells

Biochim Biophys Acta -Mol Cell Biol Lipids . 2014 Jan 13 ; 1841(4) 514 | DOI : 10.1016/j.bbalip.2014.01.002

  • A. Rebollo

Crystal structure and putative substrate identification for the Entamoeba histolytica low molecular weight tyrosine phosphatase

Mol Biochem Parasitol. . 2014 Feb 15 ; 33 193(1) | DOI : 10.1016/j.molbiopara.2014.01.003

  • A.S. Linford

Heat Stabilization of the Tissue Proteome: A New Technology for Improved Proteomics

J Proteome Res . 2009 Feb 01 ; 8(2) 974 | DOI : 10.1021/pr8006446

  • M. Svensson

Tyrosine phosphatase SHP-1 in oxidative stress and development of allergic airway inflammation.

Am J Respir Cell Mol Biol . 2008 Apr 25 ; 39(4) 412 | DOI : 10.1165/rcmb.2007-0229OC

  • Y. Cho

Poliovirus entry into human brain microvascular cells requires receptor-induced activation of SHP-2.

EMBO J . 2007 Aug 23 ; 26(17) 4016 | DOI : 10.1038/sj.emboj.7601831

  • C.B. Coyne

Liver kinase B1 expression promotes phosphatase activity and abrogation of receptor tyrosine kinase phosphorylation in human cancer cells.

J Biol Chem . 2013 Nov 27 ; 289(3) 1639 | DOI : 10.1074/jbc.M113.500934

  • I.S. Okon

Two ancient bacterial-like PPP family phosphatases from Arabidopsis are highly conserved plant proteins that possess unique properties.

Plant Physiol. . 2011 Oct 05 ; 157(4) 1778 | DOI : 10.1104/pp.111.182493

  • R. Uhrig

References

A microassay for ATPase

Anal Biochem . 1988 Mar 01 ; 169(2) 312 | DOI : 10.1016/0003-2697(88)90290-4

  • RD. Henkel

Biochemical characterization and deletion analysis of recombinant human protein phosphatase 2Cα

Biochem J . 1996 Dec 15 ; 320(3) 801 | DOI : https://doi.org/10.1042/bj3200801

  • AE. Marley
  • et al

Potent Reversible Inhibitors of the Protein Tyrosine Phosphatase CD45

J Med Chem . 2001 May 02 ; 44(11) 1777 | DOI : https://doi.org/10.1021/jm000447i

  • RA. Urbanek
  • et al