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Assay-kits

SensoLyte® Generic MMP Assay Kit Colorimetric - 1 kit

$461.00
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  • Cat.Number : AS-72095
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Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion as well as other diseases is well known.
The SensoLyte® Generic MMP Assay Kit can be used to detect the activity of a variety of MMPs, including MMP-1, 2, 3, 7, 8, 9, 12, 13, and 14 or for high throughput screening of MMPs’ inducers and inhibitors. The substrate is a thiopeptolide that is cleaved by the MMPs, releasing a sulfhydryl group, which can be detected with a color-developing thiol-reactive agent, 4,4’-dithiodipyridine or Ellman’s Reagent at 412 nm using a microplate reader. The assays are performed in a convenient 96-well microplate format.

Specifications

Packaging
Kits components
  • Component A: MMP colorimetric substrate: 10 mM, 100 mL Component B: Reference standard: 10 mM, 10 mL Component C: Assay Buffer: 20 mL Component D: MMP inhibitor: 2 mM, 10 mL Component E: Trypsin: 1 mg/mL, 100 mL Component F: Trypsin inhibitor: 10 mg/mL, 100 mL Component G: Stop Solution: 5 mL
Properties
Absorbance (nm)
  • 412
Storage & stability
Storage Conditions
  • Store all components at -20°C. Store component C at 4°C and protect from the light. Component G can be at room temperature for convenience. Aliquot Components E and F as needed to avoid freeze-thaw cycles.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • nanogram
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Inhibition of endogenous dentin matrix metalloproteinases by ethylenediaminetetraacetic acid

J Endod . 2011 Nov 03 ; 38 62 | DOI : 10.1016/j.joen.2011.09.005

  • J. Thompson

The Inhibitory Effects of Quaternary Ammonium Methacrylates on Soluble and Matrix-bound MMPs

J Dent Res . 2011 Apr 01 ; 90 535 | DOI : 10.1177/0022034510389472

  • A. Tezvergil-Mutluay

The inhibitory effect of polyvinylphosphonic acid on functional MMP activities in human demineralized dentin

Acta Biomater . 2010 May 23 ; 6 4136 | DOI : 10.1016/j.actbio.2010.05.017

  • A. Tezvergil-Mutluay

The inhibitory effects of various ions released from S-PRG fillers on dentin protease activity

Dent Mater J . 2023 Jan 31 ; 42(1) 99 | DOI : 10.4012/dmj.2022-141

  • I. Salim
  • et al

Effect of Sodium Fluoride on the endogenous MMP Activity of Dentin Matrices

J Nat Sci. . 2015 Jun 05 ; 1(6) e118 | DOI : 26052548

  • MG. Brackett
  • et al

Stabilization of dentin matrix after cross-linking treatments, in vitro

Dent Mater . 2014 Feb 01 ; 30(2) 227 | DOI : 10.1016/j.dental.2013.11.007

  • DL. Scheffel
  • et al