Assay-kits

SensoLyte® OPA Protein Quantitation Kit Fluorimetric - 1 kit

$300.00
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  • Cat.Number : AS-71015
  • Availability :
    In stock
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The OPA protein quantitation kit employs the OPA reagent for rapid and sensitive protein quantitation in solution. In general, the condensation reaction of OPA with amino-containing compounds requires the coexistence of thiol compound (such as 2-mercaptoethanol) that usually has strong unpleasant odor. AnaSpec’s OPA protein quantitation assay kit uses a proprietary formulation that only contains odorless chemicals. The OPA-based fluorometric protein assay is more sensitive than the absorbance-based methods. The kit functions well in the presence of lipids and detergents, the substances that interfere with many other protein determination methods. AnaSpec’s OPA assay kit has been shown to give faster and more sensitive detection of peptides and proteins. The kit is best used to determine proteins in the range from 3 ug/mL to 250 ug/mL.

Specifications

Packaging
Kits components
  • Component A: OPA: 350 µL Component B: Reducing solution: 200 µL Component C: Assay buffer: 20 mL Component D: BSA standard: 1 mg/mL, 500 µL
Properties
Absorbance (nm)
  • 338
Emission (nm)
  • 455
Storage & stability
Storage Conditions
  • Store all kit components at -20°C.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 3-250 ug/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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