IMG-EN-AS-72217-01.jpg
Assay-kits

SensoLyte® Rh110 Cathepsin L Assay Kit Fluorimetric - 1 kit

$582.00
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  • Cat.Number : AS-72217
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Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin L, a lysosomal endopeptidase, is a member of the papain-like family of cysteine proteinases. It is involved in the promotion of tumor cell invasion, antigen processing and turnover of intracellular and secreted proteins.
The SensoLyte® Rh110 Cathepsin L Activity Assay Kit provides a convenient assay for screening Cathepsin L inhibitors or assay of enzyme activity using a fluorogenic substrate. Upon cleavage by Cathepsin L, this substrate generates the Rh110 (rhodamine 110) fluorophore with a bright green fluorescence that can be detected at excitation/emission=490/520 nm. The longer wavelength spectra and higher extinction coefficient of Rh110 provide greater sensitivity and less interference from other reaction components.

Specifications

Packaging
Kits components
  • Component A: Rh110 Cathepsin L substrate, Ex/Em=490 nm/520 nm upon cleavage: 600 µM, 50 µL Component B: Rh110 fluorescence reference standard, Ex/Em=490 nm/520 nm: 600 µM, 10 µL Component C: Cathepsin L, human recombinant: 0.1 mg/mL, 10 µL Component D: Assay Buffer: 20 mL Component E: Cathepsin L inhibitor: 100 µM, 10 µL Component F: DTT: 1 M, 200 µL
Chemistry
UniProt number
  • P07711
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host

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Citations

Characterization of a Novel Cysteine Protease Inhibitor from Poultry Red Mites: Potential Vaccine for Chickens

Vaccines (Basel) . 2021 Dec 13 ; 9(12) 1472 | DOI : 10.3390/vaccines9121472

  • S. Fujisawa
  • et al

Type II alveolar cell MHCII improves respiratory viral disease outcomes while exhibiting limited antigen presentation

Nature Comm . 2021 Jun 28 ; 12 3993 | DOI : https://doi.org/10.1038/s41467-021-23619-6

  • SA. Toulmin
  • et al

In vitro evaluation of a cysteine protease from poultry red mites, Demanyssus gallinae, as a vaccine antigen for chicken

Poultry Sci . 2022 Mar 01 ; 101(3) 101638 | DOI : https://doi.org/10.1016/j.psj.2021.101638

  • T. Ariizumi
  • et al

In vitro evaluation of a cysteine protease from poultry red mites, Demanyssus gallinae, as a vaccine antigen for chickens

Poultry Sci . 2022 Mar 01 ; 101(3) 101638 | DOI : https://doi.org/10.1016/j.psj.2021.101638

  • T. Ariizumi
  • et al