Assay-kits

SensoLyte® Rh110 Furin Activity Assay Kit Fluorimetric - 1 kit

$504.00
Check your price
  • Cat.Number : AS-72256
  • Availability :
    In stock
  • Shipping conditions : Ice fees will apply

Quantity

Furin is a serine protease that belongs to the subtilisin-like proprotein convertase (PC) family. Furin catalyzes the proteolytic maturation of proprotein substrates in the secretory pathway. Furin plays an essential role in embryogenesis and homeostasis, as well as in diseases ranging from Alzheimer's disease, cancer, anthrax, to Ebola fever. Furin is essential to the maturation and viral infectivity of HIV. Furin is a potential target for drug design, especially for the inhibition of furin-dependent virus replication.
The SensoLyte® Rh110 Furin Assay Kit provides a convenient assay for screening of enzyme inhibitors or for continuous assay of furin activity using a fluorogenic substrate. Upon cleavage by furin, this substrate generates the Rh110 (rhodamine 110) fluorophore with bright green fluorescence that can be detected at excitation/emission=490 nm/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from other reaction components. The detection limit can reach as low as 0.02 ng/mL.

Specifications

Packaging
Kits components
  • Component A: Rh110 furin substrate, Ex/Em=490/520 nm upon cleavage: 0.6 mM, 50 µL Component B: Rh110, fluorescence reference standard, Ex/Em=490/520 nm: 0.6 mM, 10 µL Component C: Recombinant human furin: 10 µg/mL, 10 µL Component D: 2X Assay Buffer: 20 mL Component E: Inhibitor: 0.1 mM, 10 µL
Chemistry
UniProt number
  • P09958
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 0.02 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host

You may also be interested in the following product(s)

References

Furin at the cutting edge: From protein traffic to embryogenesis and disease

Nature Rev Mo Cell Biol . 2002 Oct 01 ; 3 753

  • G. Thomas

Human furin is a calcium-dependent serine endoprotease that recognizes the sequence Arg-X-X-Arg and efficiently cleaves anthrax toxin protective antigen.

J Biol Chem . 1992 Aug 15 ; 267(23) 16396 | DOI : https://doi.org/10.1016/S0021-9258(18)42016-9

  • SS. Molloy
  • et al

Structure of the unliganded form of the proprotein convertase furin suggests activation by a substrate-induced mechanism

PNAS . 2016 Oct 04 ; 113(40) 11196 | DOI : https://doi.org/10.1073/pnas.1613630113

  • S.O. Dahms

Structural homology between the human fur gene product and the subtilisin-like protease encoded by yeast KEX2.

Nucleic Acids Res. . 1990 Feb 11 ; 18(3) 664 | DOI : 10.1093/nar/18.3.664

  • A.M. Van den Ouweland
  • et al

Sequence requirements for precursor cleavage within the constitutive secretory pathway.

J. Biol. Chem. . 1992 Apr 01 ; 267(12) 8270 | DOI : https://doi.org/10.1016/S0021-9258(18)42437-4

  • T. Watanabe
  • et al